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保存染色体重塑器SMARCAD1与人类和老鼠的TFIIIC和建筑蛋白相互作用
Parysatis Sachs1,2, Philipp Bergmaier1,3, Katrin Treutwein1
1Institute of Molecular Biology and Tumor Research, Philipps University Marburg, 35043 Marburg, Germany.
Genes
|September 28, 2023
概括
在哺乳动物细胞中,SMARCAD1直接与TFIIIC相互作用,TFIIIC是关键的基因组组织者. 这种保守的伙伴关系表明了SMARCAD1.
科学领域:
- * 分子和细胞生物学
- * 基因组学和表观遗传学
背景情况:
- *SMARCAD1 (也称为HELLS或SNF2H) 是一种参与脊椎动物转录调节,异色素维护,DNA修复和复制的蛋白质.
- * SMARCAD1在这些基本细胞过程中的作用背后的精确分子机制在很大程度上仍未被阐明.
研究的目的:
- * 确定SMARCAD1的新型相互作用伙伴,并阐明它在哺乳动物细胞中的功能.
- * 调查SMARCAD1与转录因子TFIIIC之间的功能关系.
- * 探索SMARCAD1在已知功能之外的基因组组织中的作用.
主要方法:
- * 在老鼠和人类细胞模型中进行内源性共免疫沉测定,以确定SMARCAD1相互作用伙伴.
- *纯化蛋白相互作用研究证实SMARCAD1和TFIIIC之间的直接结合.
- *在小鼠胚胎干细胞 (ESC) 中进行基因表达分析,以评估SMARCAD1对TFIIIC功能的影响.
- *对TFIIIC和SMARCAD1在ESC中的全基因组分布分析.
- *分析多能和分化的哺乳动物细胞中的SMARCAD1结合伙伴.
主要成果:
- * 一般转录因子TFIIIC被确定为小鼠和人类细胞中原生SMARCAD1的直接相互作用伙伴.
- *这种SMARCAD1-TFIIIC相互作用在各种哺乳动物细胞类型中存在,包括体细胞和多能细胞.
- *SMARCAD1对于TFIIIC作为小鼠ESC中的RNA聚合酶III转录因子的作用并不重要.
- *在ESC中观察到TFIIIC和SMARCAD1的显著基因组分布,SMARCAD1没有被活性tRNA基因丰富.
- * SMARCAD1被发现与哺乳动物细胞中的染色体结构蛋白,如凝聚素,拉米尼B和DDX5有关.
结论:
- *这项研究揭示了哺乳动物细胞中SMARCAD1和TFIIIC之间的直接和保守的相互作用.
- *SMARCAD1在RNA聚合酶III转录中的作用在小鼠ESC中似乎是不可缺少的,这表明了替代功能.
- * 证据表明,SMARCAD1通过与建筑蛋白相互作用,参与哺乳动物基因组组织,扩大其已知的细胞功能.
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