在SARS-CoV-2人类受体基因中的多态性与跨种族的可变疾病结果有关
Theolan Adimulam1, Thilona Arumugam1, Anushka Naidoo2
1School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa.
Genes
|September 28, 2023
概括
ACE2和NRP1的遗传变异与COVID-19的严重程度和病毒载量有关,观察到种族差异. 具有特定基因型的非洲人表现较差,突出了疾病易感性的遗传因素.
科学领域:
- 遗传学和传染病的研究
- 分子生物学和病毒学.
背景情况:
- 人类遗传因素显著影响传染病结果,包括COVID-19.
- 在SARS-CoV-2受体/共受体基因中的特定多态性,如ACE2,TMPRSS2,NRP1和CD147,都与COVID-19的严重程度有关.
研究的目的:
- 研究SARS-CoV-2受体/共受体基因 (ACE2,TMPRSS2,CD147,NRP1) 中特定多态的作用,影响COVID-19的严重程度和病毒载量.
- 确定在南非的非洲,欧洲和南亚人群中基因型频率和与COVID-19结果的关联.
- 探索SARS-CoV-2病毒载量的种族变异及其与特定遗传变异的相关性.
主要方法:
- 从南非的非洲,欧洲和南亚血统的COVID-19阳性个体的冷样本的基因定型.
- 在ACE2 (rs2285666),TMPRSS2 (rs12329760),CD147 (rs8259) 和NRP1 (rs10080) 中对多态的分析.
- 使用滴滴数字PCR测量ACE2表达和SARS-CoV-2病毒载量.
主要成果:
- ACE2遗传变体rs2285666与COVID-19严重程度存在关联,特别是在非洲人群中 (CC基因型与更严重的结果相关).
- 与欧洲和南亚亚裔相比,非洲裔个体的SARS-CoV-2病毒载量明显较低.
- 多态 rs2285666 (ACE2) 和 rs10080 (NRP1) 与增加的SARS-CoV-2病毒载量和特定种族的更糟糕结果显著相关.
结论:
- 遗传变异rs2285666和rs10080有助于南非各族群的COVID-19严重程度和病毒载荷的差异.
- 这项研究为COVID-19结果中观察到的种族差异提供了遗传基础.
- 研究结果强调了研究潜在治疗点和定制疫苗接种策略的遗传因素的重要性.
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