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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jul 15, 2025

Purification of Transcripts and Metabolites from Drosophila Heads

Purification of Transcripts and Metabolites from Drosophila Heads

Published on: March 15, 2013

使用基于RNA-Seq的转录组研究来研究亨廷顿病中的神经元退化.

Nela Pragathi Sneha¹, S Akila Parvathy Dharshini¹, Y-H Taguchi²

¹Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India.

|September 28, 2023

概括

亨廷顿病 (HD) 涉及亨廷丁基因的CAG重复扩张,影响运动功能. 这项研究将遗传变异与原发动皮层的基因表达变化联系起来,揭示了免疫细胞基因对于减少HD中神经元死亡至关重要.

关键词:

布罗德曼地区 4 区亨廷顿病是亨廷顿氏病的一种疾病.功能互动网络互动网络组织特异性网络分析分析.变量效应的变量效应.

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Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Published on: August 3, 2018

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Published on: December 10, 2021

相关实验视频

Last Updated: Jul 15, 2025

Purification of Transcripts and Metabolites from Drosophila Heads

Purification of Transcripts and Metabolites from Drosophila Heads

Published on: March 15, 2013

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Published on: August 3, 2018

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Published on: December 10, 2021

科学领域:

神经科学是一个神经科学.
遗传学遗传学是一个
分子生物学分子生物学

背景情况:

亨廷顿病 (HD) 是一种进展性神经退行性疾病,由亨廷丁 (HTT) 基因的CAG重复扩张引起.
运动功能障碍,包括胆发病和心力衰竭,是主要的症状,起源于主要运动皮质 (BA4).
以前的研究集中在差异性基因表达上,但在HD BA4中,变体和基因表达之间的联系仍然未得到充分探索.

研究的目的:

研究亨廷顿病患者初级运动皮质 (BA4) 中遗传变异和差异表达基因/转录之间的关系.
识别影响基因表达的潜在调节变异,miRNA目标和新基因.
了解HD中神经退行症所涉及的功能网络和途径.

主要方法:

变体识别和与基因/转录量化的关联.
预测变体对监管活动的影响,包括miRNA-目标相互作用.
同表达网络和功能互动网络分析.

主要成果:

在BA4区域中发现了与差异性基因表达相关的变异.
证明了许多变体调节基因表达并影响miRNA-目标相互作用.
同表达网络突出显示了新型基因,功能网络突出显示了囊泡介导的运输通路.

结论:

免疫细胞表达的基因被认为对减轻亨廷顿病中神经元死亡至关重要.
这种统一的方法可以更深入地了解HD中神经元退化的遗传基础.
这些发现表明,与HD的免疫反应和囊泡运输相关的潜在治疗点.