酵母 Lsm 亲细胞突变突变体在自中表现出缺陷
Benedetta Caraba1, Mariarita Stirpe1, Vanessa Palermo1
1Department of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Roma, Italy.
International journal of molecular sciences
|September 28, 2023
概括
切断酵母中必不可少的LSM4基因会导致细胞死亡标记物,并阻断自. 这表明LSM4在自过程的后期阶段起着至关重要的作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 酵母遗传学 酵母遗传学
背景情况:
- LSM4是一种重要的酵母基因,对通过LSM复合体进行mRNA处理,稳定性和翻译至关重要.
- 之前的研究表明,在S. cerevisiae Lsm4无菌株中表达了截断的K. lactis LSM4,恢复了生命力,但诱导了细胞死亡标志物.
- 这些标记物包括活性氧物种 (ROS) 和氧化RNA积累.
研究的目的:
- 为了研究Saccharomyces cerevisiae中LSM4的C端Q/N丰富域的功能.
- 确定LSM4在超越mRNA代谢的细胞过程中的作用,特别是自.
- 描述与截断S. cerevisiae LSM4基因相关的表型.
主要方法:
- 基因切断:创建一个切断的S. cerevisiae LSM4基因,类似于之前研究的K. lactis基因.
- 表型分析:评估细胞活力,活性氧物种 (ROS) 水平和转化酵母菌株中氧化RNA积累.
- 自试验:在饥饿和拉帕治疗条件下评估Sclsm4Δ1突变体的自过程.
主要成果:
- 切断S. cerevisiae LSM4基因重复了与K. lactis切断基因观察到的细胞死亡表型.
- 这种Sclsm4Δ1突变体在自过程中表现出显著的阻断.
- 这种突变者对饥饿和低剂量拉巴胺过敏,并积累了与自相关的结构.
结论:
- LSM4直接参与了自的调节,可能是在该过程的后期阶段.
- 在LSM4的C端Q/N丰富的域似乎对适当的自功能很重要.
- LSM4的失调影响了mRNA代谢和自途径,突出了其多方面的细胞作用.
更多相关视频
10:39A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
Published on: September 17, 2020
6.3K
11:39Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Published on: July 21, 2017
31.3K
相关概念视频
Delivery Pathways to the Lysosome
6.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.6K
Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K
Autophagy
4.3K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.3K
Lysosomal Hydrolases
3.8K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.8K
The Intrinsic Apoptotic Pathway
6.6K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
