人类心脏的两步转录组分析揭示了外宫嗅觉受体的广泛和对疾病敏感的表达
Sadia Ashraf1, O Howard Frazier2, Sylvia Carranza2
1Department of Internal Medicine, Division of Cardiology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
International journal of molecular sciences
|September 28, 2023
概括
研究人员在人类心脏中发现了160多个嗅觉受体 (ORs),其中38个表现出心力衰竭的改变. 这些发现表明ORs可能在心脏病的发病过程中发挥作用,并提供新的治疗点.
科学领域:
- 心脏病学 心脏病学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- G蛋白结合受体 (GPCRs) 调节心脏功能,是心脏病治疗的目标.
- 异位嗅觉受体 (ORs),一种GPCR,在非鼻腔组织中发现,并影响心脏代谢.
研究的目的:
- 为了对人心中的嗅觉受体 (OR) 基因表达进行分析.
- 为了识别由于缺血性心肌病导致的心力衰竭失调的ORs.
- 探索改变ORs在心力衰竭病原发生中的作用.
主要方法:
- 从心力衰竭患者 (n=18) 和非失败对照 (n=4) 的左心室组织的转录组分析.
- 实时PCR阵列量化了372个不同的OR转录.
- RNA测序确定了全球心脏基因表达.
主要成果:
- 在人类心脏中发现了160个嗅觉受体 (OR).
- 38个OR被发现在心力衰竭中受到差异调节.
- 同表达分析涉及 mitochondrial 功能障碍,细胞外矩阵重塑和炎症中的失调 ORs.
结论:
- 人类的心脏表达了大量的嗅觉受体 (OR).
- 心力衰竭与特定ORs的改变表达有关.
- 调节失调的ORs可能会导致心力衰竭的发病,呈现出潜在的治疗点.
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