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未成熟和体内成熟的马匹积卵细胞复合体的转录组特征

Alejandro de la Fuente^1,2, Charles Scoggin³, Etta Bradecamp³

¹Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.

International journal of molecular sciences

|September 28, 2023

概括

这项研究揭示了在体外成熟 (IVM) 过程中马类卵细胞和累积细胞的关键基因表达变化. 了解这些转录基因动态可以改善体外胚胎生产 (IVP) 和马类辅助生殖技术 (ART).

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
动物科学动物科学

背景情况:

试管成熟 (IVM) 对于马类胚胎生产至关重要,但面临着具有可变成功率的挑战.
优化IVM需要对控制卵细胞成熟和发育能力的分子机制有更深入的了解.

研究的目的:

通过比较卵细胞和累积细胞中的基因表达来研究马类卵细胞成熟期间的转录组动态.
为了识别未成熟与体外成熟卵细胞及其周围的累积细胞中的差异表达基因 (DEGs) 和相关的生物途径.

主要方法:

收集了积卵细胞复合体,并将其分为不成熟和体外成熟 (MII) 组.
在两个组的分离卵细胞 (OC) 和累积细胞 (CC) 上进行了RNA测序.
进行了差异基因表达分析和基因本体学 (GO) 丰富分析.

主要成果:

在OC中共鉴定了13,918个转录,其中538个DEG位于不成熟和MII卵细胞之间.
在CC中,确定了13,104个转录,其中871个是DEGs.
在GO分析中,DEG与OC和GTPase活性中的核成熟,细胞外矩阵组织和CC中的原三元体有关. FSHR和LHCGR在CC中显示了差异性的表达.

结论:

这项研究为马类卵细胞成熟提供了基础的转录组数据集.
已识别的DEG和途径为改善马的IVM效率和发育能力提供了洞察力.
这些发现可以提高马匹辅助生殖技术 (ART) 和临床管理.

关键词:

累积细胞中的累积细胞.马类的马类动物.在体外胚胎生产.在体外卵子成熟的过程.卵子细胞卵子细胞转录组 (transcriptome) 是一个转录组.

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