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在Pinus massoniana中克隆PmMYB6及其功能分析

Yuan He¹, Qingqing Hao¹, Peizhen Chen¹

¹State Key Laboratory of Tree Genetics and Breeding, Key Open Laboratory of Forest Genetics and Gene Engineering of National Forestry & Grassland Administration, Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.

International journal of molecular sciences

|September 28, 2023

概括

这项研究确定Pinus massoniana MYB6 (PmMYB6) 是植物发育中的关键调节剂. 植物中PmMYB6的过度表达增强了体生长,素和黄酸盐含量,影响了甲代谢.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
生物化学生物化学

背景情况:

烯胺是影响生长,发育和环境相互作用的必需植物化合物.
类似MYB的转录因子是植物生长和类生物合成的关键调节者.

研究的目的:

为了功能性地描述Pinus massoniana基因PmMYB6,一个R2R3-MYB转录因子.
研究PmMYB6在烯胺代谢流量分布中的作用.

主要方法:

定量PCR (qPCR) 用于确定PmMYB6在Pinus massoniana组织中的表达模式.
在异质系统 (烟草和) 中过度表达PmMYB6,以评估其功能影响.

主要成果:

PmMYB6在P.massoniana的花朵,体和体中显示出高的表达.
过度表达PmMYB6的转基因植物表现出扩大了体,增加了红素和黄酸盐含量.
在转基因植物中观察到多个基因通路酶基因的升级.

结论:

PmMYB6在烯基通路内的代谢流量调节中发挥着重要作用.
这项研究提供了关于P. massoniana中flavonoid和lignin生物合成之间的代谢流量的调节的见解.
这些发现为P. massoniana.分子育种策略提供了基础.

关键词:

转录因子MYB转录因子松树 (Pinus massoniana) 是一种松树.在PmMYB6中.黄类化合物黄类化合物红色的线性蛋白质 (lignin) 是一种烯基代谢途径的发生.

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