相关实验视频
Updated: Jul 15, 2025

08:01
The Soft Agar Colony Formation Assay
Published on: October 27, 2014
111.9K
新型长非编码RNA (lncRNA) 转录AL137782.1 通过积极调节LMO7促进正常肺上皮细胞的迁移
Ying Zhang1,2, Weili Wang1,2, Chunchun Duan1,2
1Life Science School, Ningxia University, Yinchuan 750001, China.
International journal of molecular sciences
|September 28, 2023
概括
这项研究确定了一种新的长非编码RNA (lncRNA),AL137782.1,它调节肺上皮细胞迁移. 虽然AL137782.1增强了正常细胞的迁移,但它的表达在肺癌中减少.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 长非编码RNA (lncRNAs) 在生物过程中起着至关重要的作用,但它们的功能往往不清楚.
- 新型lncRNA ENSG00000261553,特别是它的转录AL137782.1,在微生物暴露后在肺上皮细胞中被上调,并在肺腺癌中表现出差异性表达.
- 这些观察表明,AL137782.1在正常的肺细胞功能和疾病病理生理学中都存在作用.
研究的目的:
- 为了研究lncRNA AL137782.1在肺上皮细胞中的功能.
- 确定AL137782.1在细胞迁移中的调节作用及其与LMO7.7的关系.
- 了解AL137782.1表达在肺癌中的含义.
主要方法:
- 用于表达特征的生物信息学分析.
- 光在位杂交 (FISH) 和亚细胞分离用于局部化.
- 快速放大cDNA末端 (RACE),以确认转录长度.
- 在体外测试以评估细胞迁移和LMO7表达.
主要成果:
- 一个4401 nt lncRNA,AL137782.1,在肺上皮细胞中被识别并定位.
- AL137782.1积极调节LMO7.7的mRNA和蛋白质表达.
- 在体外,AL137782.1的过度表达增加了正常和肺腺癌细胞的迁移.
- 重要的是,与相邻组织相比,AL137782.1表达在肺癌中显著下降.
结论:
- AL137782.1-LMO7轴主要促进正常肺上皮细胞中的细胞迁移.
- 肺癌中AL137782.1的表达减少表明它不会通过这种迁移途径驱动癌症的进展.
- 这项研究为人类肺上皮细胞迁移机制提供了新的见解,并提供了增强肺损伤后细胞迁移的潜在策略.
相关概念视频
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Non-LTR Retrotransposons
11.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.6K
Regulated mRNA Transport
6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Regulation of Expression at Multiple Steps
942
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
942
Regulation of Expression Occurs at Multiple Steps
22.8K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.8K

