紫色和绿色开花的中国白菜的比较转录组分析和BrMYB114基因的功能分析
Mei Fu1, Juxian Guo1, Kang Tang1,2
1Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China.
International journal of molecular sciences
|September 28, 2023
概括
研究人员确定了BrMYB114基因,该基因调节紫色开花的中国卷心菜中的安东生物合成. 这一发现揭示了这种蔬菜鲜的茎颜色背后的分子机制.
科学领域:
- 植物分子生物学 植物分子生物学
- 农业科学 农业科学
- 生物化学 生物化学
背景情况:
- 开花的中国白菜 (Brassica rapa var. 这种白菜有很多种) 菜 (Parachinensis) 是一个在中国南部很受欢迎的蔬菜.
- 安托素是重要的抗氧化剂,有助于某些品种的紫色,使相关基因对质量特征至关重要.
研究的目的:
- 为了确定负责紫色开花的中国白菜中安东生物合成的基因.
- 为了阐明背后紫色茎色彩的分子机制.
主要方法:
- 使用RNA-sequencing (RNA-seq) 进行转录组分析,对两种鲜花的中国白菜品种进行了分析,这些品种具有对比的茎颜色.
- 生物信息学分析以确定差异表达基因 (DEG) 和丰富的途径.
- 在Nicotiana benthamiana中候选基因的异质表达.
主要成果:
- 鉴定了6811个差异表达基因 (DEG),包括295个转录因子.
- 烯胺,黄和黄醇以及黄胺生物合成途径在紫色品种中得到显著丰富.
- 与氨酸生物合成相关的25个DEG在紫色茎中表达更高,BrMYB114被确定为关键候选基因.
结论:
- BrMYB114在调节开花的中国白菜中安托氨酸生物合成方面发挥着至关重要的作用.
- 这些发现提供了对这种蔬菜紫色茎发育的分子基础的重要见解.
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