基因组和转录组洞察MIKC型MADS盒基因在Carica papaya中的演化和分歧
Yunsu Dai1,2, Yu Wang1,2,3, Liwang Zeng4
1Sanya Nanfan Research Institution of Hainan University, Sanya 572025, China.
International journal of molecular sciences
|September 28, 2023
概括
研究人员在番茄中发现了24个MIKC型MADS盒 (II型) 基因,揭示了基因重复和损失塑造了它们的进化. 这些基因表现出不同的表达模式,表明植物发育和应激反应中的不同作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- MIKC型MADS-box (II型) 基因对于花器官的形成和植物的繁殖发育至关重要.
- 目前尚缺少Carica的全基因组数据和对这些基因的转录基因分析.
研究的目的:
- 为了识别和表征卡里卡木瓜基因组中的II型基因.
- 通过转录基因分析研究这些基因的进化历史和功能作用.
主要方法:
- 在Carica papaya中对24个II型基因的全基因组识别.
- 遗传学分析以确定进化关系和基因重复事件.
- 在152个样本中进行大规模的转录组分析,以评估基因表达模式.
- 基因共同表达网络分析和qRT-PCR验证.
主要成果:
- 二十四个II型基因被确定并分为13个子类,有遗传损失和重复的证据.
- 基因重复显著促进了MIKC型基因扩张,重复的对显示表达分歧.
- 在各种组织中观察到不同的表达模式,以及对生物和非生物压力的反应.
- 特定的基因TT16-3和AGL8与雄性花的发育和种子发芽有关.
结论:
- 番茄的II型MADS盒基因谱是由基因丢失和重复事件所塑造的.
- 基因重复在产生II型基因之间的功能多样性方面发挥着至关重要的作用.
- 这些基因表现出专门的表达特征,表明它们在发育和应激反应中扮演着不同的角色.
- 这项研究提供了关于Carica papaya中MIKC型基因的演变和功能的基础见解.
相关概念视频
Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
Evolutionary Relationships through Genome Comparisons
5.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.8K
Gene Duplication and Divergence
6.2K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.2K
Synteny and Evolution
3.3K
John H. Renwick first coined the term “synteny” in 1971, which refers to the genes present on the same chromosomes, even if they are not genetically linked. The species with common ancestry tend to show conserved syntenic regions. Therefore, the concept of synteny is nowadays used to describe the evolutionary relationship between species.
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral...
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral...
3.3K
Fruit Development, Structure, and Function
22.4K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
22.4K
Eukaryotic Evolution
34.9K
The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
34.9K


