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在食道状细胞癌和腺癌中,G蛋白结合雌激素受体GPER1的不同表达模式
Jingshi Liu1, Yongdong Niu2, Bin Zhang1
1Department of Histology and Embryology, Shantou University Medical College, Shantou 515041, China.
International journal of molecular sciences
|September 28, 2023
概括
在食道癌中,G蛋白结合雌激素受体1 (GPER1) 的表达不同. 在食道腺癌 (EAC) 中,GPER1的上调,抑制其生长,但在食道状细胞癌 (ESCC) 中下调.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
背景情况:
- 食道癌表现出明显的性别偏见,特别是食道腺癌 (EAC).
- 在食道癌中,G蛋白结合雌激素受体1 (GPER1) 的作用和表达尚不清楚.
- GPER1与各种与性别有关的癌症有关.
研究的目的:
- 在食道癌中调查GPER1mRNA和蛋白质表达水平.
- 确定GPER1在不同组织学类型的食道癌症中的功能作用.
- 探索GPER1表达和患者存活率之间的相关性.
主要方法:
- 使用GEPIA和Oncomine数据库对GPER1mRNA水平的元分析.
- 在EAC和ESCC组织微阵列中检测GPER1蛋白的免疫组织化学检测.
- 在体外实验中,使用食道细胞系上的GPER1激动剂 (G1),抗剂 (G15) 和siRNA进行实验.
主要成果:
- 在整体食道癌中,GPER1 mRNA表达减少,与生存率差相关.
- 在EAC及其前体巴雷特食道中,GPER1RNA和蛋白质过度表达,与差异化不良相关.
- 与正常组织相比,ESCC中GPER1蛋白的下调.
- 在GPER1阳性ESCC细胞中,GPER1主激素G1抑制了增殖并促进了细胞亡,而对抗剂G15或siRNA则逆转了效应.
结论:
- 在EAC中,GPER1的表达明显高于ESCC.
- GPER1可能在不同的食道癌亚型的发病过程中发挥不同的作用.
- GPER1信号可能是一个潜在的治疗目标,特别是在EAC.
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