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人眼表面微生物组及其与干眼疾病中的眼蛋白质组的关联

Irina Schlegel¹, Claire M F De Goüyon Matignon de Pontourade¹, Joel-Benjamin Lincke¹

¹Department of Ophthalmology, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.

International journal of molecular sciences

|September 28, 2023

概括

干眼病 (DED) 涉及眼睛表面微生物群和眼蛋白之间的复杂相互作用. 这项研究揭示了DED患者的微生物和蛋白质配置的改变,提供了新的治疗点.

科学领域:

眼科医生眼科眼科
微生物学微生物学
蛋白质组学是指蛋白质组学.

背景情况:

干眼病 (DED) 是一种普遍存在的眼睛表面疾病,其致病性不明,治疗方法有限.
新出现的证据将眼睛表面微生物组 (OSM) 和液组成与DED病原体联系起来,可能是通过炎症途径.

研究的目的:

在DED患者中确定与人类和微生物眼蛋白相关的OSM的独特组成和功能特征.
研究患有DED的个体的OSM和撕裂蛋白质组的变化.

主要方法:

整个元基因组枪测序在40个眼和结膜拭子上进行.
进行了OSM的分类学和功能分析.
进行了微生物丰富度和人类眼蛋白之间的相关性分析.

主要成果:

动态细菌和蛋白质细菌是最丰富的类;Propionibacterium acnes是主要的物种.
在DED患者的结膜样本中,Corynebacterium tuberculostearicum的数量更为丰富.
参与氨基酸和四聚醇生物合成的基因在对照中得到了丰富;Acinetobacter johnsonii的丰富度与人眼蛋白如粘素-16.6相关.

结论:

在DED患者中,OSM和蛋白质组的组成和功能特征发生了显著的变化.
这些发现可以指导开发针对DED的新型干预疗法.

关键词:

染色体学并联质谱学质谱学干眼疾病是眼睛干燥的疾病.眼睛表面的微生物组撕裂蛋白质的蛋白质组整个元基因组的猎枪测序.

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