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银素诱导的与伤口愈合相关的反应涉及皮肤上生长因子类效应在角质细胞中
Kyung-Jong Won1, Rami Lee2, Sun-Hye Choi3
1Department of Physiology and Medical Science, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
International journal of molecular sciences
|September 28, 2023
概括
一种人参化合物银素通过激活表皮生长因子 (EGF) 受体和刺激肝素结合性EGF类生长因子 (HB-EGF) 在角质细胞中的释放来促进皮肤伤口愈合.
科学领域:
- 皮肤病学 皮肤病学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 皮表皮生长因子 (EGF) 受体信号驱动着角质细胞的增殖和迁移.
- 肝素结合EGF类似生长因子 (HB-EGF) 激活EGF受体,促进角质细胞的功能.
- 丁素是人参衍生的溶酸 (LPA) 受体连接体,表现出皮肤伤口愈合特性,但其机制尚不清楚.
研究的目的:
- 为了研究EGF受体 (EGFRs) 在HaCaT角质细胞中因丁介导的伤口修复中的作用.
- 为了阐明参与金宁皮肤愈合作用的信号通路.
主要方法:
- 细胞增殖试验 (基于水溶性四盐的试验).
- 细胞迁移试验 (修改的博伊登室试验).
- 蛋白质分析 (免疫阻塞) 来评估受体的激活和表达.
主要成果:
- 丁素显著增加了HaCaT细胞中的EGF受体激活.
- 丁素诱导的EGF受体酸化被LPA和EGFR抑制剂 (Ki16425,erlotinib) 降低.
- 丁素刺激的角质细胞增殖和迁移被EGFR抑制剂 (erlotinib,AG1478) 阻断.
- 丁素增强了HB-EGF的表达和释放,而EGFR抑制剂则抑制了这种表达和释放.
结论:
- 丁的伤口愈合作用是由EGF受体的激活作用.
- 银素通过 EGFR 激活和 HB-EGF 释放的途径促进角质细胞的增殖和迁移.
- EGFR信号传递和HB-EGF之间的相互作用对于金通在皮肤修复中的治疗潜力至关重要.
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