在从慢性压力男性衍生的后代中,miR-29a被下调
Marta F Riesco1, David G Valcarce1, Alba Sellés-Egea1
1INDEGSAL, Cell Biology Area, Molecular Biology Department, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.
International journal of molecular sciences
|September 28, 2023
概括
斑马鱼的父性压力通过改变microRNA-29a水平来改变后代的发育. 这种分子破坏导致后代的生存率和化率下降.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 父亲的压力暴露越来越多地被认为有可能影响后代的表型.
- 父亲压力影响后代发育的机制,特别是在分子水平上,需要进一步阐明.
研究的目的:
- 为了研究父亲慢性压力会对斑马鱼后代产生负面影响的假设.
- 为了识别分子变化,特别是microRNAs (miRNAs),以及由父亲压力导致的后代的生理变化.
主要方法:
- 斑马鱼雄性接受了为期21天的慢性压力方案.
- 用RNA测序 (RNA-seq) 来识别来自应激的幼虫中差异表达的小非编码RNA.
- 对差异表达的miRNA的验证在幼虫,精子,丸和大脑组织中进行.
主要成果:
- 一个单一的微RNA,miR-29a,在来自父亲压力男性的幼虫中被发现显著下调.
- 这种miR-29a的下调是垂直观察到的,这表明压力影响的传播到未暴露的后代.
- 受影响的后代表现出降低的生存率和化率,以及对发育,形态发生和神经发生的潜在影响.
结论:
- 父亲的慢性压力可以垂直传播给后代,诱导像miR-29a放松调节这样的分子变化.
- 观察到的后代的分子变化与发育受损和生命能力下降有关.
- 这些发现突出了父亲遗传的慢性压力的新型分子后果,影响后代的健康和发育.
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