在SARS-CoV-2感染期间血中蛋白质修饰的简短调查
Agnieszka Gęgotek1, Neven Zarkovic2, Biserka Orehovec3
1Department of Analytical Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-069 Bialystok, Poland.
International journal of molecular sciences
|September 28, 2023
概括
严重的COVID-19改变了参与表细胞分裂和血小板脱粒的血蛋白. 脂质过氧化产物如4-HNE和MDA添加物增加,可能预测感染结果.
科学领域:
- 生物化学 生化学
- 免疫学 免疫学 免疫学
- 病理学 病理学 病理学
背景情况:
- 严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 感染可能导致呼吸衰竭和高死亡率.
- 了解严重的COVID-19分子病理学至关重要,即使在流行病结束后也是如此.
研究的目的:
- 为了比较血蛋白质组,专注于脂质过氧化产品添加物,在COVID-19幸存者,已故个体和健康对照中.
- 为了确定区分不同COVID-19结果的潜在生物标志物.
主要方法:
- 分析了来自COVID-19幸存者的血样本 (n=10),已故的个人 (n=10) 和健康的对照 (n=10).
- 蛋白质组分析的重点是蛋白质表达和通过脂质过氧化产品的修饰,包括4-氨酸 (4-HNE),氨酸 (MDA) 和15-deoxy-12,14-prostaglandin J2 (15d-PGJ2).
- 统计分析,包括ANOVA与错误发现率校正,用于识别显著改变的蛋白质.
主要成果:
- COVID-19显著改变了调节外细胞和血小板脱粒的蛋白质,死者患者的变化更为明显.
- 在COVID-19和已故组中观察到4-HNE和MDA adducts的水平增加.
- 15d-PGJ2 adducts在幸存者中增加,但在已故患者中显著减少,影响像p53和STAT3.3这样的蛋白质.
结论:
- 这项研究表明,COVID-19患者的血中形成了脂质代谢产品-蛋白质添加物.
- 这些引证在幸存者和死者之间有很大差异.
- 血脂过氧化添加物可以作为SARS-CoV-2感染过程和结果的预测剂.
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