鉴定SH2域含有蛋白3C作为二基酸酶3的新型假定相互作用体
Mihaela Matovina1, Ana Tomašić Paić1, Sanja Tomić1
1Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička Cesta 54, 10000 Zagreb, Croatia.
International journal of molecular sciences
|September 28, 2023
概括
二乙酶3 (DPP3) 与SH2域含蛋白3C (SH2D3C) 相互作用. 这种通过质谱学识别的新型相互作用表明DPP3在细胞信号通路中的新角色.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 二乙酶3 (DPP3) 是一种依赖的外酶,参与调节NRF2-KEAP1氧化应激通路.
- 含SH2域蛋白3C (SH2D3C),也称为NSP3,是一种调节细胞粘附,迁移和免疫反应的适应蛋白.
研究的目的:
- 确定二二酶3 (DPP3) 的新型相互作用伙伴.
- 描述DPP3和SH2域含蛋白3C (SH2D3C) 之间的相互作用.
主要方法:
- 细胞培养中氨基酸的稳定同位素标记与质谱学 (SILAC-MS) 相互作用组分析相结合.
- 在活细胞中的同局部化研究和计算结合分析.
主要成果:
- 确定SH2域含蛋白3C (SH2D3C) 是与DPP3诱相互作用的猎物.
- SH2D3C通过其C端 Ras GEF类域与DPP3结合.
- 这种相互作用在细胞质和膜中得到证实,并观察到同局部化.
结论:
- 这项研究揭示了Dipeptidyl peptidase 3 (DPP3) 和SH2域含蛋白3C (SH2D3C) 之间的新型相互作用.
- 这些发现表明DPP3在由SH2D3C调节的细胞过程中可能具有新的作用.
- 需要进一步的研究才能充分阐明这种DPP3-SH2D3C相互作用的生理意义.
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