相关实验视频

Updated: Jul 15, 2025

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

转录基因和代谢分析揭示了大豆中与阴影耐受性相关的关键基因

Aohua Jiang¹, Jiaqi Liu¹, Weiran Gao¹

¹College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.

International journal of molecular sciences

|September 28, 2023

概括

这项研究揭示了关键的基因和途径,涉及到大豆叶在阴影下的发展. 已识别的候选基因为改善作物的阴影耐受机制提供了洞察力.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

大豆 (Glycine max) 是一种重要的作物,但其生长和发育受到光线的可用性,特别是阴影条件的显著影响.
控制大豆叶在阴影下发育的精确分子机制在很大程度上是未知的,这阻碍了改善阴影耐受性的努力.

研究的目的:

阐明大豆叶在阴影条件下发育的调节机制.
确定与大豆阴影耐受性相关的差异表达基因 (DEG),关键通路和候选基因.

主要方法:

在自然光和阴影条件下,大豆叶的转录组和代谢组测序.
生物信息分析包括KEGG丰富,转录因子查和加权基因共同表达网络分析 (WGCNA).
使用定量实时聚合酶链反应 (qRT-PCR) 验证候选基因.

主要成果:

鉴定了265个差异表达基因 (DEGs),其中144个是下调的,121个是上调的.
基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因.
通过WGCNA发现了34个差异表达的转录因子 (DETF),10个通过WGCNA验证的核心枢纽基因和12个潜在参与阴影耐受性的候选基因.

结论:

这项研究为大豆阴影耐受性的分子基础提供了新的见解.
已确定的候选基因,如ATP-PRT2,PEPC,IAA17和PAP,为基因解剖和分子育种提供了有价值的目标,以提高大豆的阴影耐受性.

关键词:

代谢组分析分析阴影宽容度阴影宽容度豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆转录组分析转录组分析

更多相关视频

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Photoreceptors and Plant Responses to Light

Photoreceptors and Plant Responses to Light

Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.