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miR-30a-3p 调节自在小鼠乳腺内卷的自

Lei Tian¹, Shancheng Guo¹, Zhiye Zhao¹

¹Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.

International journal of molecular sciences

|September 28, 2023

概括

微RNA-30a-3p (miR-30a-3p) 通过向与自相关的12 (Atg12) 通过抑制自来调节乳腺内置. 这一发现澄清了哺乳期到发育期过渡期间的分子机制.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
发育生物学发展生物学

背景情况:

乳腺重塑在哺乳周期中至关重要.
乳腺内置涉及广泛的上皮细胞自.
微RNA在调节细胞过程中起着重要的作用.

研究的目的:

调查miR-30a-3p在乳腺发育中的作用.
阐明 miR-30a-3p 调节自的分子机制.
了解miR-30a-3p在发育过程中对乳腺结构的影响.

主要方法:

在哺乳期的miR-30a-3p表达的定量分析与涉及小鼠乳腺的量化分析.
研究小鼠乳腺上皮细胞 (MMECs) 中miR-30a-3p和与自相关的基因 (例如Atg12,Atg5) 之间的调控关系.
实验性操纵包括miR-30a-3p的过度表达,ATG12的恢复,ATG5的淘汰,饥饿和拉帕米辛治疗.

主要成果:

与哺乳期相比,miR-30a-3p的表达在乳腺发育过程中明显较低.
miR-30a-3p直接准并负面调节与自相关的12 (Atg12),从而抑制自.
过度表达miR-30a-3p会抑制自,并在发育过程中破坏乳腺结构.

结论:

miR-30a-3p在乳腺发育过程中作为自的关键调节剂.
miR-30a-3p/Atg12轴是一个关键的分子机制,控制乳腺在哺乳期和发育期之间的重塑.

关键词:

在Atg1212中使用.自自是自的过程.进化,进化,进化,进化,进化,进化.哺乳周期的哺乳周期乳腺腺是乳腺的一种.这是一个小RNARNA.

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