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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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对COVID-19代谢学的元分析确定了生物标志物的稳定性变化
Anthony Onoja1, Johanna von Gerichten2, Holly-May Lewis3
1School of Health Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford GU2 7XH, UK.
International journal of molecular sciences
|September 28, 2023
概括
这项研究使用代谢学验证了COVID-19生物标志物,发现一个由9个生物标志物组成的小组显示了最强大的表现. 这些发现强调了对未来传染病进行可再生生物标志物研究的需要.
科学领域:
- 生物化学 生物化学
- 传染病研究 传染病研究
- 代谢学 代谢学 代谢学
背景情况:
- 随着COVID-19的流行,诊断和治疗的生物标志物发现加速了.
- 随着COVID-19变得流行,其生物标志物研究的经验教训对于未来的病原体准备至关重要.
- 评估拟议的生物标志物面板的稳定性对于临床应用至关重要.
研究的目的:
- 评估先前识别的研究衍生生物标志物面板的稳定性,以区分COVID-19患者.
- 评估一个众包面板的COVID-19代谢生物标志物经常被引用在文献 (2020-2023).
- 通过使用独立的定量代谢学数据集来验证生物标志物小组的性能.
主要方法:
- 利用向和定量液体染色学-质谱学 (LC-MS) 代谢学数据集进行独立验证.
- 与独立数据集对比,测试了五个不同的研究衍生生物标记面板.
- 根据文献频率评估了一个众包小组.
主要成果:
- 表现最好的小组由九种生物标志物组成:乳酸,谷氨酸,酸,氨酸,氨酸,β-氨酸,鸟类素,酸,胆和素.
- 这个由9个生物标志物组成的小组在独立数据集中获得了F1分数0.76和AUROC0.77.
- 与原始发现相比,具有较少代谢物的面板在独立队列中显示出较弱的统计学意义和较差的表现.
结论:
- 在独立人群中进行强有力的验证对于基于代谢学的生物标志物面板的临床实用性至关重要.
- 该研究强调了在代谢学分析中评估可重复性的重要性,以获得可靠的诊断.
- 研究结果为未来的新兴传染病生物标志物研究策略提供了信息,强调了队列独立验证.
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