二甲酸增强了Streptococcus pyogenes中酸结合反感核酸的抗菌作用
Corina Abt1, Lisa Marie Gerlach1, Jana Bull1
1Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany.
乙酸 (PyB) 通过改善细菌吸收,增强了Streptococcus pyogenes中与细胞透 (CPP) 结合的反感性核酸 (PNA) 的抗菌活性. 在Streptococcus pneumoniae中没有观察到这种效应.
科学领域:
- 开发抗菌药物 开发抗菌药物
- 细菌膜转位的转位是细菌膜的转位.
- 酸核酸化学 酸核酸化学
背景情况:
- 反感性核酸 (PNA) 显示出抗菌潜力,但需要携带者才能进入细菌.
- 细胞透 (CPPs) 促进PNA转移到细菌中.
- 像pyrenebutyrate (PyB) 这样的疏水性对应物可以增强化物膜的透性.
研究的目的:
- 调查pyrenebutyrate (PyB) 在支持CPP结合的反意义PNAs转移到细菌中的有效性.
- 评估PyB对PNA对Streptococcus pyogenes和Streptococcus pneumoniae的抗菌活性的影响.
主要方法:
- 反意义 PNA 与 CPP 的结合.
- 用CPP-PNA治疗S. pyogenes和S. pneumoniae,有或没有PyB.
- 对细菌生长抑制和吸收的评估 (使用K8-FITC).
主要成果:
- 在S. pyogenes中,PyB显著增强了CPP结合的反感 PNA 的抗菌活性,特别是与抗-gyrA PNA 的抗菌活性.
- 对于CPP-PNAs,没有观察到PyB对S. pneumoniae的显著影响.
- 在PyB前化改善了K8-FITC在S. pyogenes,大肠杆菌和K. pneumoniae中的吸收.
结论:
- 在S. pyogenes中,PyB促进了CPP结合的反意义PNAs在细菌膜上的转移.
- PyB是增强基于PNA的抗微生物药物对某些格拉姆阳性细菌的疗效的有希望的辅助剂.
- 似乎PyB的作用机制是增强膜转位,具有菌株特异性的效应.
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