在家禽中Listeria monocytogenes的活力和毒性
Sarah Panera-Martínez1,2, Rosa Capita1,2, Camino García-Fernández2
1Department of Food Hygiene and Technology, Veterinary Faculty, University of León, 24071 León, Spain.
Microorganisms
|September 28, 2023
概括
李斯特菌单细胞菌在家禽中普遍存在,通过文化依赖和文化独立的方法检测到. 这种病原体具有众多的毒性因子和抗生素耐药性,强调需要严格的卫生在家禽加工,以防止人类菌病.
科学领域:
- 食品微生物学 食品微生物学
- 食品安全 食品安全
- 细菌学 细菌学是一门学科.
背景情况:
- 李斯特菌 (Listeria monocytogenes) 是一种重要的食物传播病原体.
- 禽类是食物传播疾病的常见载体.
- 准确检测和表征L. monocytogenes对于公共卫生至关重要.
研究的目的:
- 为了确定L. monocytogenes在家禽样本中的流行率.
- 为了比较文化依赖和文化独立的检测方法.
- 鉴定从家禽中分离的L. monocytogenes病毒性因子,生物膜形成和抗生素耐药性的特征.
主要方法:
- 文化依赖的方法:OCLA上的隔离和通过常规PCR (OCLA&PCR) 的确认.
- 独立于培养的方法:实时PCR (q-PCR) 与活力标志物 (v-PCR).
- 病毒性基因的PCR检测,生物膜的水晶紫色检测和抗生素敏感性测试.
主要成果:
- 在50%的样本中,通过OCLA&PCR检测到L. monocytogenes,而通过q-PCR检测到66.7%.
- 所有的分离物都具有6-9的毒性基因. 生物膜产量有所不同,其中33.3%无法形成生物膜.
- 对几种抗生素观察到抗生素耐药性/敏感性降低的高患病率,包括牛素,西普罗素和尼托夫兰.
结论:
- 这项研究强调了L. monocytogenes在家禽中的显著存在,具有相当大的毒性潜力和抗生素耐药性.
- 与传统方法相比,像q-PCR这样的文化独立方法的检测率更高.
- 在家禽加工过程中,严格的卫生做法对于减轻人类菌病的风险至关重要.
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