活菌 AJ01 通过L-谷氨酸促进致病性
Ya Li1,2, Weibo Shi1,2, Weiwei Zhang1,2
1Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315832, China.
Microorganisms
|September 28, 2023
概括
过多的L-胺酸 (L-Glu) 通过增强细菌的运动性,增加了海中的Vibrio splendidus的毒性. 保持L-Glu平衡对于宿主防御这种病原体至关重要.
科学领域:
- 海洋生物学 海洋生物学
- 病原体与宿主之间的相互作用
- 生物化学 生物化学
背景情况:
- 斑杆菌 (Vibrio splendidus) 是一种影响海洋无脊椎动物的重要病原体,特别是海黄瓜 (Apostichopus japonicus).
- 在压力下,L-Glutamic 酸 (L-Glu) 含量上升,并成为 V. splendidus AJ01.01 的重要营养物质.
研究的目的:
- 调查外源L-胺酸 (L-Glu) 对海黄健康和V. splendidus毒性的影响.
- 阐明L-Glu对宿主-病原体相互作用影响的基础分子机制.
主要方法:
- 在暴露于L-Glu后,对细胞活力,组织完整性和海黄瓜死亡率的分析.
- 转录基因分析 (GO和KEGG) 来识别差异表达的基因.
- 实时逆转录酶PCR验证基因表达变化,特别是fliC基因.
主要成果:
- 10毫米的L-Glu显著降低了细胞的活力,增加了海黄的死亡率,导致组织损伤.
- 0.1毫米的L-Glu对宿主生存和组织完整性的影响很小.
- 转录组分析揭示了参与信号和膜形成的基因的显著上调和内细胞化,而 lysosomal 途径被下调.
- 同时暴露于L-Glu和V. splendidus加剧了胆固醇细胞损失和死亡率.
- L-Glu 调节了 V. splendidus fliC 基因,增强了细菌的运动性.
结论:
- 外源的L-Glu,特别是高度的L-Glu,会损害海黄的免疫防御,增加对V. splendidus感染的易感性.
- 过多的L-Glu通过增加细菌的运动性来促进V. splendidus的毒性,这凸显了在宿主-病原体界面保持L-Glu平衡的重要性.
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