通过针对性下一代测序16S rRNA和ITS技术对两种番茄种类的微生物多样性的表征
Rukayat Abiola Abdulsalam1, Oluwatosin Ademola Ijabadeniyi1, Errol D Cason2
1Department of Biotechnology and Food Science, Durban University of Technology, Durban 4000, South Africa.
Microorganisms
|September 28, 2023
概括
这项研究揭示了储存期间果和圆形番茄中不同的微生物群落. 下一代测序显示,细菌多样性通常超过真菌多样性,随着时间的推移而发生的转变.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 基因组学就是基因组学.
背景情况:
- 微生物腐烂显著影响番茄 (Solanum lycopersicum) 的营养和经济价值.
- 关于番茄在腐烂过程中的微生物群落的数据有限,之前的研究依赖于文化依赖的方法.
研究的目的:
- 在储存期间对南非两种番茄品种 (果和圆形) 的微生物多样性进行纵向表征.
- 用下一代测序来比较西红中的细菌和真菌社区动态.
主要方法:
- 雇员针对的高通量下一代测序.
- 在储存1,6和12天后分析了果和圆形番茄品种的微生物群落.
主要成果:
- 在整个储存过程中,细菌群体的多样性始终高于真菌群体.
- 随着时间的推移,各品种的微生物多样性有所不同;圆形番茄最初显示出更高的多样性,而果番茄在第12天有更大的多样性.
- 主导的细菌系包括蛋白质菌,菌菌和细菌菌; 主导的真菌系是阿斯科米科塔和巴西迪奥米科塔. 普遍存在的属包括Pantoea,Klebsiella,Hanseniaspora,Stemphylium和Alternaria. 这三种类型的植物有着很大的影响力.
结论:
- 该研究提供了全面的微生物多样性概况,用于储存期间的西红.
- 证实了微生物丰富性和多样性的特异性差异,影响了腐烂动态.
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