鱼皮和鱼骨中的原蛋白的分布,典型结构和自我组装特性
Xuening Zhang1, Jie Wang1, Qian Zhang1
1College of Food Science and Engineering, Ocean University of China, Sansha Road, Qingdao 266404, China.
Molecules (Basel, Switzerland)
|September 28, 2023
概括
来自鱼和滑板来源 (皮肤,骨,软骨) 的原蛋白被分析其特性和自我组装. 结果揭示了基于来源和类型的独特特征,影响了原蛋白的应用.
科学领域:
- 生物材料科学 生物材料科学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 原蛋白的来源和类型对于其多样化的应用至关重要.
- 来自鱼类的原蛋白是哺乳动物来源的有希望的替代品.
研究的目的:
- 从鱼 (皮肤和骨头) 和滑板 (皮肤和软骨) 提取的原蛋白的特征.
- 研究这些原体的结构性,热性和自组装性.
- 从每个来源中识别独特的原序列.
主要方法:
- 福里埃变换红外光谱 (FTIR) 用于分析分子结构.
- 紫外线-Vis光谱学和X射线衍射 (XRD) 对于结构性质.
- 循环二元论 (CD) 用于形状分析.
- 差分扫描热量计 (DSC) 用于测量热性能.
- 使用质谱测量进行酸序列测量.
主要成果:
- 皮肤中的原纤维显示出纵向排列,而软骨原显示出各种颜色.
- FTIR光谱证实了典型的原胺带 (A和B) 的存在.
- 特定的紫外线吸收,XRD d值和CD Rpn值观察到骨和软骨原蛋白.
- 热变质温度在皮肤和软骨原之间有所不同.
- 鱼皮肤和滑板软骨原蛋白的最佳自我组装pH值不同.
- 鉴定了鱼和滑板原蛋白的独特序列,包括I型和II型原蛋白标记物.
结论:
- 原蛋白的特性受到来源 (鱼与滑板) 和组织类型 (皮肤,骨,软骨) 的显著影响.
- 具有特征的原体表现出不同的结构,热和自组装行为.
- 确定了独特的序列,为鱼类原蛋白多样性和潜在应用提供了分子洞察力.
相关概念视频
Structural Protein Function
27.8K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
27.8K
Collagens are the Major Structural Proteins of ECM
4.3K
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. Collagen fiber is made from fibrous protein subunits linked together to form a long, straight fiber. Collagen fibers, while flexible, have great tensile strength, resist stretching, and give ligaments and tendons their characteristic resilience and strength. These fibers hold connective tissues together, even during the body's movement.
Connective tissue proper includes loose...
Connective tissue proper includes loose...
4.3K
Type IV Collagen of Basal Lamina
2.3K
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...
A type IV collagen molecule has six alpha chains which can...
2.3K
Fibril-associated Collagen
2.6K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.6K
Fibrous Proteins
2.3K
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...
2.3K
The Bone Matrix
3.2K
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
3.2K


