快速诊断用于检测T-2和HT-2毒素在麦中的比较性能
Julie Meneely1,2, Brett Greer1,2, Oluwatobi Kolawole1,2
1Institute for Global Food Security, National Measurement Laboratory: Centre of Excellence in Agriculture and Food Integrity, Queen's University Belfast, 19 Chlorine Gardens, Belfast BT9 5DL, UK.
Molecules (Basel, Switzerland)
|September 28, 2023
概括
在麦中监测T-2和HT-2菌毒素对于食品安全至关重要. Neogen Reveal® Q+ MAX套件为现场测试提供了可靠,准确和具有成本效益的解决方案,最大限度地降低了人类暴露风险.
科学领域:
- 农业科学 农业科学
- 食品安全 食品安全
- 分析化学 分析化学
背景情况:
- 麦被三甲基菌毒素 (T-2和HT-2) 污染构成了严重的食品安全风险.
- 气候变化,农业和收获后处理加剧了麦作物的真菌毒素存在.
- 有效的监测和快速测试对于限制人类接触这些有害毒素至关重要.
研究的目的:
- 评估在麦作物中检测T-2和HT-2真菌毒素的四种商业快速诊断套件的性能.
- 将这些套件与黄金标准分析方法 (液体染色学-质谱学) 进行比较.
- 确定最可靠,最准确,最实用的工业使用的测试方法.
主要方法:
- 四个商业快速诊断套件 (三个侧流装置,一个ELISA) 被测试.
- 对液体染色学与质谱学 (LC-MS) 结合的性能进行了评估.
- 进行了统计分析 (ANOVA) 和定性分析 (假阳性/假阴性率).
主要成果:
- 通过单向ANOVA评估的评估方法之间没有发现显著差异 (p=0.45).
- 虚假阴性/阳性率有所不同:第一套 (1.1%/2.2%),第二套 (7.6%/0%),第三套 (2.2%/0%),第四套 (6.5%/0%).
- 尼奥根Reveal® Q+ MAX (测试套件1) 证明了最高的可靠性,准确性和成本效益.
结论:
- Neogen Reveal® Q+ MAX套件是一个合适的,用户友好的选择,用于在麦中现场检测T-2/HT-2真菌毒素.
- 该套件的易用性不需要专门的技术技能,促进了广泛采用.
- 准确和可访问的测试对于确保食品安全和管理食品工业中真菌毒素风险至关重要.
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