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封装铁和乳酸氨酸以保护蛋白质分解,同时保持铁的协调和持续释放

Przemysław Gajda-Morszewski^1,2, Anna Poznańska¹, Cristina Yus^3,4

¹Department of Inorganic Chemistry, Faculty of Chemistry, Jagiellonian University in Kraków, Gronostajowa 2, 30-387 Krakow, Poland.

Nanomaterials (Basel, Switzerland)

|September 28, 2023

概括

在Eudragit RS微粒中封装乳酸铁素 (Lf) 可以提高它向肠道的输送. 这种优化的系统可以保护和铁的全-乳酸铁素免受胃部的降解,从而保持其治疗性质.

科学领域:

生物化学生物化学
材料科学材料科学材料科学
药理学药理学是指药理学的学科.

背景情况:

乳酸 (Lf) 是一种牛奶糖蛋白,具有抗微生物,抗病毒,抗癌和免疫调节作用.
将结构完整的Lf有效地输送到肠道对于实现其治疗潜力至关重要.
Lf的铁结合能力是其生物功能的核心.

研究的目的:

为了优化铁和乳酸铁素 (holoLf) 在Eudragit RS微粒中的封装.
为了保护holoLf在胃肠道,特别是胃的蛋白质分解环境中的降解.
在模拟生理条件下评估微粒中holLf的释放概况和结构完整性.

主要方法:

使用Eudragit RS聚合物对holoLf进行微封装,以形成微粒 (MP).
微粒子形态的表征,捕获效率和负载能力.
使用模拟的胃和肠液 (欧洲药典标准) 的体外释放研究.
在释放后评估hololf的结构完整性和铁结合性质.

主要成果:

微粒子实现了高捕获效率 (90-95%) 和负载能力 (9.7%),具有最佳的球形形态.
在模拟的胃条件下,MPs证明了holoLf对酶消化和离子铁释放的部分保护.
在模拟的胃条件下进行预化,释放了40%的全体Lf,其结构保持不变,并保持了33%的铁和和铁清理活动.

结论:

欧德拉吉特RS微粒提供了一个可行的系统,可以在胃肠道过境期间保护乳酸铁素.
封装过程保留了holoLf的结构完整性和功能性质.
这种输送系统有望提高乳酸铁的治疗效果.

关键词:

乳酸铁氨酸 (lactoferrin) 是一种乳酸铁氨酸.这些微粒是微粒.提供蛋白质的蛋白质输送.蛋白质封装封装是一种蛋白质封装.

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