人类牛奶通过持有器消毒或通过高水静压处理进行灭菌导致小鼠的肠道效应不同
Lionel Carneiro1,2, Lucie Marousez3, Matthias Van Hul2,4,5
1INSERM U1220, Institut de Recherche en Santé Digestive (IRSD), Université Paul Sabatier, Toulouse III, CHU Purpan, Place du Docteur Baylac, CS 60039, CEDEX 3, 31024 Toulouse, France.
Nutrients
|September 28, 2023
概括
高水位压力 (HHP) 处理供体乳 (DM) 增强小鼠的肠道屏障完整性. 这种方法可能对早产婴儿的肠道成熟有好处,与Holder消毒 (HoP) 相比.
科学领域:
- 胃肠病学 胃肠病学
- 儿童营养学 儿童营养学
- 食品科学 食品科学 食品科学
背景情况:
- 人体牛奶银行 (HMB) 为早产婴儿消毒供体牛奶 (DM),主要使用持有者消毒 (HoP).
- 高水静压 (HHP) 处理是一种新兴的DM替代绝育方法.
- 处理HHP的DM对肠道健康的影响需要进一步研究.
研究的目的:
- 为了比较慢性口服服用HoP或HHP加工的供体乳对肠道屏障完整性的影响.
- 评估HHP加工的供体牛奶对肠道微生物群组成和短链脂肪酸 (SCFA) 生产的影响.
主要方法:
- 成年小鼠每天接受HoP或HHP加工的捐赠者牛奶口服测量,持续7天.
- 通过体内FITC-dextran测定来评估肠道透性.
- 分析了结膜和结肠中紧密结节和粘膜的mRNA表达,以及状SCFA和微生物群组成.
主要成果:
- 在小鼠中,HHP加工的供体牛奶显著降低了肠道通透性.
- 在HHP-DM给药后,紧结基因 (Ocln,Cdh1) 和Muc2的Ileal表达增加.
- 在HoP-DM和HHP-DM组之间没有观察到内SCFA或微生物群组成的显著差异.
结论:
- 供体牛奶的HHP加工在体内加强了肠道屏障的完整性.
- HHP加工不会对肠道微生物群或SCFA生产产生不利影响.
- HHP提供了一个潜在的有益的替代品HoP用于消毒捐赠者的牛奶,支持在早产婴儿的肠道成熟.
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