两种商用血液稳定试剂作为冠状病毒的强大失活剂
Joseph J O'Connor1, Lynden Voth1, Jeremiah Athmer2
1Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA.
Pathogens (Basel, Switzerland)
|September 28, 2023
概括
两种血液采集管试剂,即Cell-Free DNA BCT® (cfDNA) 和Cyto-Chex® BCT (CytoChex),有效地禁用了冠状病毒 (CoV) 并保存了它们的RNA7天,有助于检测和测试.
科学领域:
- 病毒学 病毒学
- 生物技术是生物技术.
- 公共卫生 公共卫生
背景情况:
- 由于持续循环和频繁爆发,冠状病毒 (CoV) 构成了持续的全球健康威胁.
- 需要有效的工具来监测和预防冠状病毒的传播,包括SARS-CoV-2.
研究的目的:
- 为了评估两种血液采集管 (BCT) 试剂,即 Cell-Free DNA BCT® (cfDNA) 和 Cyto-Chex® BCT (CytoChex) 的有效性,用于禁用冠状病毒 (CoV) 和稳定其RNA.
- 评估这些试剂在涉及CoV的诊断和研究应用中使用的潜力.
主要方法:
- 测试cfDNA和CytoChex试剂对三种不同的冠状病毒无活化的能力:小鼠肝炎病毒 (MHV),人类冠状病毒OC-43 (OC-43) 和SARS-CoV-2.
- 评估两种试剂在7天内对病毒RNA的稳定性.
- 用试剂处理的缓冲和全血样本中的病毒失活率与对照样本的比较.
主要成果:
- cfDNA和CytoChex试剂都在18小时内显著降低了感染性病毒水平,低于检测极限,有时早在3小时内.
- 在测试条件下,CytoChex表现出更强大的抗病毒活性,与cfDNA相比,实现更快的无活化.
- 尽管病毒迅速失活,但两种试剂都有效地保持了病毒RNA完整性长达7天.
- 这两种试剂都加速了血液样本中的病毒失活,与未经处理的对照组相比.
结论:
- cfDNA和CytoChex试剂显示出在保持病毒RNA的同时,在禁用传染性冠状病毒方面具有显著的潜力.
- 这些BCT试剂可以成为收集样本,运输和储存在诊断和研究环境中的宝贵工具,用于Cov检测和分析.
- 这些发现支持使用cfDNA和CytoChex来开发改善的监测和管理冠状病毒爆发的策略.
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