在巴西患有发性肝炎的患者中检测人类佩吉病毒-1和基因定型
Anielly Sarana da Silva1, Gabriel Montenegro de Campos1, Marcia Guimarães Villanova2
1Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14051-140, SP, Brazil.
Pathogens (Basel, Switzerland)
|September 28, 2023
概括
人类佩吉病毒-1 (HPgV-1) 经常在发性肝炎患者中检测到. 这项研究确定了基因型2作为这些严重肝病例中占主导地位的HPgV-1菌株.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 病毒学 病毒学
- 传染性疾病 传染性疾病
背景情况:
- 爆发性肝炎是一种严重的疾病,肝功能迅速下降和脑病变.
- 之前的转基因组学研究表明,在发性肝炎患者中,人类佩吉病毒-1 (HPgV-1) 的存在显著.
- 在严重的肝病中HPgV-1的作用和患病率在很大程度上仍然未被描述.
研究的目的:
- 在被诊断患有 fulminant 肝炎的患者中确定人类病毒-1 (HPgV-1) 的频率和分子特征.
- 为了确定这个患者队列中的HPgV-1循环基因型.
- 调查HPgV-1与严重肝功能障碍的潜在关联.
主要方法:
- 来自 fulminant 肝炎患者的血样本被分析以检测HPgV-1 RNA的存在.
- 实时和嵌套PCR技术用于病毒检测.
- 对阳性样本进行了基因定型测试,以确定HPgV-1亚型.
主要成果:
- 在44.4%的分析样本中检测到人类病毒-1 (HPgV-1) RNA (27个中12个).
- 阳性样本显示平均周期值 (Ct) 值为28.5 (±7.3).
- 所有检测到的HPgV-1菌株都属于2型基因型,并确定了2A和2B亚型.
结论:
- 人类佩吉病毒-1 (HPgV-1) 在发性肝炎患者中普遍存在,主要是作为基因型2.
- 需要进一步的研究,以阐明HPgV-1在发性肝炎和其他临床疾病的发病过程中的潜在作用.
- 了解HPgV-1患病率和基因型对于评估其临床意义至关重要.
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