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在肠道中的牛内皮T淋巴细胞的表征

Akanksha Hada¹, Lei Li¹, Anmol Kandel¹

¹Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.

Pathogens (Basel, Switzerland)

|September 28, 2023

概括

牛内皮T淋巴细胞 (T-IELs) 与人类和小鼠相比,表现出独特的特征,特别是它们的T细胞受体 (TCR) 表达和CD8共受体配置文件. 这项研究为牛群中这些重要的免疫细胞提供了第一个详细的特征.

科学领域:

免疫学免疫学免疫学
兽医科学兽医科学兽医科学
细胞生物学细胞生物学

背景情况:

内皮质T淋巴细胞 (T-IELs) 对于粘膜免疫至关重要,防御病原体并维持肠道平衡.
虽然在人类和小鼠中进行了广泛的研究,但牛T-IEL概况在很大程度上仍未表征.

研究的目的:

通过流细胞计学来表征牛T-IELs的表型和功能标记.
为了将牛T-IEL子集的组成与人类和小鼠中发现的子集进行比较.

主要方法:

牛肠内皮质淋巴细胞的流动细胞计分析.
在T-IELs上识别和量化T细胞受体 (TCR) 和共受体 (CD4,CD8) 的表达.
激活 (CD69) 和淋巴细胞指向标 (CD62L) 的评估,以及细胞因子的产生 (IFNγ,IL17A,TGFβ1).

主要成果:

牛T-IEL显示TCRγδ+和TCRαβ+T细胞的分布几乎相等 (每个约为50%).
大多数牛TCRγδ+T-IEL是CD8- (超过60%),与小鼠不同.
鉴定出了不同的TCRαβ+亚群,包括CD4+CD8αβ+ (约. 20%) 和缺乏TCRαβ+CD8αα+细胞,不同于人类和小鼠的形状.
牛T-IEL表达高CD69和低CD62L,类似于其他物种,并产生低水平的炎症和调节性细胞因子.

结论:

与人类和小鼠T-IEL相比,牛T-IEL表现出独特的组成,特别是TCRγδ+细胞内的CD8种群和TCRαβ+CD4+CD8αβ+细胞的存在.
这些发现为了解牛T-IEL在肠道健康和疾病中的功能奠定了基础.
需要进一步的研究来探索牛T-IELs在对肠道病原体和屏障完整性的反应中的作用.

关键词:

CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD4 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD8 CD9 CD8 CD8 CD8 CD8 CD9 CD8 CD9 CD8 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 CD9 是一个字体的字体的字体是什么意思 IFNγ IFNγ 的意思是在 IL17A 中.这就是T-IELs.这就是TCRαββ.这就是TCRγδδ.在TGFβ1中.这里是牛群.内皮层T淋巴细胞的内皮层T淋巴细胞

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