相关实验视频

Updated: Jun 20, 2026

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Published on: May 30, 2017

在小鼠乳腺腺癌细胞中CAIX表达的分子共振成像

Claudia Quattrociocchi¹, Alberto Mangia¹, Silvio Aime²

¹Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Turin, 10126 Turin, Italy.

Pharmaceuticals (Basel, Switzerland)

|September 28, 2023

概括

研究人员开发了一种新型的MRI探针,针对乳腺癌中的碳酸无水酶IX (hCAIX). 这种探针对表达hCAIX的细胞具有很高的选择性和敏感性,提供了一个有前途的诊断工具.

科学领域:

在瘤学瘤学.
生物医学成像技术生物医学成像技术
生物化学生物化学

背景情况:

碳酸无水酶IX (hCAIX) 对于细胞外瘤pH调节至关重要,并且在包括乳腺癌在内的各种癌症中过度表达.
hCAIX的表达与瘤进展,转移和治疗耐药性相关,使其成为一个重要的诊断和治疗点.
针对hCAIX的细胞外蛋白糖类 (PG) 域,为癌症成像提供了一种新的策略.

研究的目的:

开发一种有针对性的磁共振成像 (MRI) 探针,用于检测碳酸无水酶IX (hCAIX).
创建一个能够与hCAIX的细胞外PG域进行特定相互作用的纳米探针.
为了评估这个探针在体外乳腺癌模型中的有效性.

主要方法:

基于脂质体的纳米探针 (LIP_PepC) 的设计和合成,装有Gd轴承对比剂.
对hCAIX PG域特异的基互动因子在脂质体表面的结合.
使用已知表达hCAIX的小鼠乳腺癌细胞系 (TS/A) 进行体外试验.

主要成果:

LIP_PepC纳米探针在对表达hCAIX的TS/A细胞的MRI成像中表现出高选择性和灵敏性.
探测器成功地准了hCAIX的细胞外PG域.
核磁共振成像结果证实了探头在癌症模型中检测hCAIX存在的能力.

结论:

开发的LIP_PepC纳米探针是针对hCAIX表达瘤的基于MRI的向诊断的一个有前途的工具.
这种方法促进了hCAIX的非侵入性成像,可能改善早期癌症检测和治疗监测.
该策略具有在癌症诊断中体内转化和临床应用的巨大潜力.

关键词:

CAIXIX CAIXIX 这是一个很好的例子.Gd-对比剂是一种对比剂.这就是为什么MRI是MRI.乳腺癌乳腺癌乳腺癌脂质体是一种脂质体.分子成像分子成像技术

更多相关视频

Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad

Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad

Published on: January 20, 2019

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Published on: January 20, 2022

相关概念视频

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...