相关实验视频

Updated: Jul 15, 2025

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

微小的细胞外囊泡的光追踪 in vivo

Yanxia Chen¹, Yinghong Shi^1,2, Zhimin Tao^1,2

¹Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, China.

|September 28, 2023

概括

用于标记小细胞外囊泡 (sEVs) 的光染料对小鼠的瘤积累有不同的影响. 研究人员建议避免单一染料染色,以期未来对sEV生物命运的研究.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
纳米技术纳米技术

背景情况:

小型细胞外囊泡 (sEVs) 对于细胞间通信至关重要.
在体内跟踪SEV对于了解其治疗潜力至关重要,特别是在癌症中.
光成像是跟踪sEVs的常见方法,但染料选择可能会影响结果.

研究的目的:

研究不同有机和无机光染料对胃癌模型中SEV跟踪的影响.
为了比较使用各种染料标记的sEVs的生物分布,循环和瘤积累.
为了评估细胞起源对sEV行为的影响,当标记相同的染料时.

主要方法:

人类带 (Huc) 中介细胞干细胞 (MSC),HEK293T细胞和HGC细胞衍生的sEVs被可见或近红外光染料染色.
被标记的sEVs被给予人类胃癌异种移植的小鼠模型.
使用光成像监测了sEVs的循环,主要器官的生物分布,以及依赖时间的瘤积累.

主要成果:

不同的光染料导致sEVs独特的瘤积累模式.
来自不同细胞系的sEVs在使用相同的染料时表现出类似的血液循环和瘤积累概况.
光成像证实了对sEVs的可靠追踪,但观察到对生物分布的染料特异性影响.

结论:

光染料的选择显著影响了sEVs观察到的生物分布和瘤积累.
虽然来自各种来源的SEV显示了可比的体内行为,但染料诱导的文物可能会使解释复杂化.
关于sEV生物命运的未来研究应该考虑多种染料或替代标签策略,以避免单种染料膜染色的潜在工件.

关键词:

光是一种光.胃癌胃癌是一种胃癌.接近红外的窗户.小动物成像成像技术小小的细胞外囊泡.

更多相关视频

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

相关概念视频

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...