来自俄罗斯的桃病毒A和小桃病毒1的第一份报告和完整基因组表征
Sergei Chirkov1, Anna Sheveleva1, Svetlana Tsygankova2
1Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
Plants (Basel, Switzerland)
|September 28, 2023
概括
这项研究报告了俄罗斯首次检测到桃病毒A (CVA) 和小桃病毒1 (LChV1),这些病毒在各种Prunus杂交品种中被发现. 这些发现扩大了我们对病毒分布和石头水果遗传多样性的理解.
科学领域:
- 植物病毒学 植物病毒学
- 园艺科学 园艺科学
- 基因组学就是基因组学.
背景情况:
- 病毒性疾病对石头果实 (Prunus spp.) 有重大影响. 产量,质量和寿命.
- 桃病毒A (CVA) 和小桃病毒1 (LChV1) 是影响普鲁努斯作物的常见和经济重要病毒.
研究的目的:
- 测序,组装和表征来自俄罗斯普鲁努斯杂交物种的CVA和LCHV1分离体的全长基因组.
- 确定这些病毒分离的遗传关系和遗传多样性.
主要方法:
- 使用高通量测序来获得完整的病毒基因组.
- 进行了植物遗传学分析,以了解已识别的病毒分离物的进化关系.
主要成果:
- 在P. cerasifera × P. armeniaca杂交物中确定了CVA,该杂交物与非桃分离物分类.
- 在复杂的三混合体中检测到LCHV1 (孤立的Stepnoe),这表明P. cerasifera和P. brigantiaca是潜在的宿主.
- LChV1 Stepnoe分离体与希腊分离体的核酸相同度为77.3%,可能代表一个新的族群.
结论:
- 这项研究提供了俄罗斯CVA和LChV1的第一份报告.
- 这些发现扩大了对这些石果病毒的地理分布和遗传多样性的知识.
- 突出了新的普鲁努斯物种作为LCHV1宿主的潜力.
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