通过酶性水解获得的奇托寡合物的功能性质
Dominika Kulig1, Żaneta Król-Kilińska1, Łukasz Bobak1
1Department of Functional Food Development, Faculty of Food Science and Biotechnology, Wroclaw University of Environmental and Life Sciences, 37 Chelmonskiego St., 51-630 Wroclaw, Poland.
Polymers
|September 28, 2023
概括
使用细胞酶或基托酶对基托的酶化水解产生具有不同性质的基托醇糖化物 (COS). 所得到的分数显示了溶解度,热稳定性和对大肠杆菌的抗菌活性的差异.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 酸盐 (CH) 是一种多功能生物聚合物,具有潜在的应用.
- 提高基托的功能性质对于其更广泛的使用至关重要.
- 酶性水解提供了一种可控的方法来生产素衍生物.
研究的目的:
- 使用细胞酶 (CL) 和基多酶 (CS) 来从基多中产生基多酸盐 (COS).
- 为了比较从不同的酶处理和隔离分数中获得的COS的功能性质.
- 为了评估酶解水解对酸盐可溶性,热稳定性和生物活性的影响.
主要方法:
- 使用CL和CS进行基托桑的酶化水解.
- 基托寡合物的分离成沉物 (HMF) 和超物 (LMF) 分数.
- 使用降低糖含量,粘度测量,CPMAS 13C NMR,热重力测量分析 (TGA) 和DPPH激素清除试验进行表征.
- 对抗大肠杆菌的抗微生物活性评估.
主要成果:
- 酶性水解通过增加降解糖含量和降低粘度得到证实.
- CPMASC 13C 核磁共振显示了不同的酶分裂机制.
- 低粉和高粉碎片的水溶性 (94.56%) 比高粉和高粉 (70.64%) 高.
- 在TGA中,LMF显示了最大的质量损失 (58.35%),这表明热灵敏度.
- 与CS相比,由CL产生的COS的聚合度较低 (DP3-7).
- 与HMF和NF (DP 3-7) 相比,LMF (DP~2) 的DPPH基因清理活性较低.
- 对大肠杆菌的抗菌活性以以下顺序增加:LMF>NF>HMF>CH.
结论:
- 细胞酶和胆酶产生具有明显特征的胆醇糖类.
- 分解显著影响COS的功能性质,包括溶解性和生物活性.
- 由此产生的COS分量显示了对大肠杆菌的不同程度的抗菌疗效.
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