在意大利流传的三角洲菌株的植物遗传学和植物动力学分析
Leonidas Salichos1, Claudia Minosse2, Ubaldo Visco-Comandini3
1Biological and Chemical Sciences, New York Institute of Technology, Manhattan, NY 10023, USA.
Viruses
|September 28, 2023
概括
肝炎三角病毒 (HDV) 基因型1,特别是1d亚型,在意大利普遍存在. 植物动力学分析显示,在20世纪50年代和80年代之间,HDV-1在拉齐奥地区多次引入,没有单一的祖先起源.
科学领域:
- 病毒学 病毒学
- 进化生物学 进化生物学
- 流行病学 流行病学
背景情况:
- 肝炎三角病毒 (HDV) 是高度可变的,在全球范围内发现了八种基因型 (HDV-1至 -8).
- 大型肝炎病毒1型 (HDV-1) 是全球最常见的,在欧洲,北美和北非普遍存在,并与不同严重程度的肝病有关.
研究的目的:
- 调查意大利拉齐奥地区的HDV菌株的遗传变异性和引入动态.
- 通过植物遗传学和植物动力学分析,了解意大利HDV-1的进化历史和起源.
主要方法:
- 来自拉齐奥 (Lazio) 的患者的50个HDV RNA阳性样本使用嵌套RT-PCR和针对HDV R0区域的测序进行了分析.
- 使用RAxML v8和贝叶斯的出生死亡模型进行了家族遗传学和家族动力学分析,并结合了HDV基因型1-8的参考序列.
主要成果:
- 在拉齐奥省,HDV-1是主要的基因型,HDV-1d亚型是最常见的,以及1b和1e亚型.
- 植物动力学分析估计HDV-1时钟速率为3.04 × 10^-4替代每位每百万年.
- 贝叶斯分析表明,从20世纪50年代末到80年代末,HDV-1在该地区的多次引入,驳斥了一个单一的共同祖先起源.
结论:
- 拉齐奥地区的高密度病毒菌株主要属于1型基因型,有多种不同的亚型在循环中.
- 在意大利,HDV-1的引入发生在几十年内,来自多个来源,反映了复杂的流行病学模式.
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