在p16-Negative Vulvar 内皮层病变中与状细胞癌相关的贝塔皮罗马病毒
Taja Lozar1,2,3, Aysenur Keske4, Racheal S Dube Mandishora5,6
1McArdle Laboratory for Cancer Research, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.
Viruses
|September 28, 2023
概括
在p16负的状状病变中检测到贝塔人类乳头瘤病毒 (β-HPVs). 这一发现表明β-HPV可能有助于 vulvar状细胞癌 (vSCC) 的发展,缺乏α-HPV (α-HPV) 关联.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 皮肤病学 皮肤病学
背景情况:
- 在约40%的病例中,状状细胞癌 (vSCC) 与阿尔法人类乳头瘤病毒 (α-HPV) 有关.
- 对α-HPV阴性vSCC的原因尚不清楚.
- 贝塔人类乳头瘤病毒 (β-HPV) 与皮肤状细胞癌 (cSCC) 有关,可能在阴道病变中发挥作用.
研究的目的:
- 调查p16阴性皮状内皮病变 (vSILs) 与vSCC相邻的β-HPV的存在和潜在作用.
- 探索vSCC发展的替代病因路径.
主要方法:
- 在与vSCC相邻的28个vSIL上进行了全面的HPV基因型鉴定.
- 对p16和p53进行了免疫组织化学和形态学审查.
- 量化PCR (qPCR) 和RNA in situ杂交用于选定的病例.
主要成果:
- 在80% (8/10) 的p16阴性vSIL中检测到β-HPV DNA.
- 在21% (3/14) 的p16阳性高等级vSIL中也发现了β-HPV DNA.
- 检测到的HPV DNA负载有显著的变化,从每细胞<1到>100个副本.
结论:
- 这项研究是首次报告β-HPVs和p16-负vSILs之间的关联.
- 这些发现表明β-HPV可能参与α-HPV阴性vSCC的病因学.
- 这些结果扩大了对部病变潜在致病原体的理解.
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