酸盐脱酶激酶1调节了人类自然杀手细胞的功能
Huirong Li1, Linghui Cheng1, Shiyue Su1
1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Anhui Province Key Laboratory of Reproductive Health and Genetics, Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, Hefei, Anhui Province, China.
American journal of reproductive immunology (New York, N.Y. : 1989)
|September 28, 2023
概括
酸盐脱酶激酶1 (PDK1) 在发生性自然杀手 (dNK) 细胞中升高,这些细胞存在于经常性自发性流产的女性中. 抑制PDK1会损害dNK细胞的功能并降低糖解,这表明PDK1在RSA中的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 生殖生物学 生殖生物学
- 细胞代谢 细胞代谢
背景情况:
- 酸盐脱酶激酶1 (PDK1) 对于免疫细胞发育至关重要.
- 对于PDK1在人类自然杀手 (dNK) 细胞中的特定作用尚不清楚.
- 复发性自发性流产 (RSA) 是一种具有复杂免疫学基础的疾病.
研究的目的:
- 为了研究PDK1在人类dNK细胞中的表达和功能,来自患有RSA的女性.
- 确定PDK1抑制对dNK细胞活动,迁移,粘附和新陈代谢的影响.
主要方法:
- 使用定量实时PCR (qRT-PCR),西斑和流动细胞测量来分析PDK1的表达.
- 用PDK1抑制剂或siRNA治疗dNK细胞以评估功能变化.
- 功能性测试包括细胞毒性 (51Cr释放试验),迁移,粘附和糖解测量.
主要成果:
- 与健康对照组相比,RSSA患者的dNK细胞中PDK1,穿孔素,Granzyme B,IFN-γ和CD107a的表达显著更高.
- 抑制PDK1导致细胞毒性标记物的表达减少和dNK细胞杀死活性降低.
- PDK1阻断增强了dNK细胞的迁移和粘附,同时降低了糖解.
结论:
- PDK1在调节人类dNK细胞功能方面发挥着重要作用.
- 增加PDK1的表达和活性可能会导致反复性自发性流产的病理生理学.
相关概念视频
The Extrinsic Apoptotic Pathway
6.4K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Inhibition of Cdk Activity
4.8K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K


