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家用洗衣剂破坏屏障完整性,并诱导老鼠和人类皮肤的炎症
Arturo O Rinaldi1, Manru Li1, Elena Barletta1
1Swiss Institute of Allergy and Asthma Research (SIAF), University Zurich, Davos, Switzerland.
Allergy
|September 28, 2023
概括
洗衣剂,包括硫酸二二 (SDS),破坏皮肤屏障. 在小鼠和人类皮肤中观察到的这种干扰可能会导致诸如阿托皮病之类的炎症性皮肤疾病.
科学领域:
- 皮肤病学 皮肤病学
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
背景情况:
- 表皮屏障功能障碍与炎症性皮肤和粘膜疾病有关.
- 之前的体外研究表明,洗衣剂可能会损害上皮质屏障的完整性.
研究的目的:
- 研究家用洗衣剂对皮肤屏障功能的体内和体外影响.
- 分析皮肤在暴露于洗剂后发生的分子变化.
主要方法:
- C57BL/6小鼠的背皮用洗衣剂处理;通过电阻谱学 (EIS) 和跨皮肤水损失 (TEWL) 评估屏障功能.
- 皮肤活检样本经过RNA测序 (RNA-seq) 和向多重蛋白质组学.
- 活体人体皮肤被用洗剂和二硫酸盐 (SDS) 处理,以测量EIS.
主要成果:
- 洗剂治疗显著降低了EIS,并在小鼠皮肤中增加了TEWL,显示了剂量依赖性.
- RNA-seq揭示了紧密结节和粘附结节的下调基因,以及上调的角质化和脂质代谢.
- 蛋白质组显示细胞粘附蛋白减少和益炎性细胞因子 (IL-6,IL-1β) 增加;人体皮肤也显示减少了EIS.
结论:
- 洗衣剂和SDS在体内和体外皮肤模型中损害了表皮屏障功能.
- 每天接触洗剂可能会破坏皮肤屏障,可能会导致亚托皮病的发展.
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