从家禽肠道中分离出来的菌,用于潜在的益生菌用途
E Carvajal1, S Contreras2, W Díaz2
1Universidad de Santander, Facultad de Ciencias Médicas y de la Salud, Instituto de Investigaciones Masira, Bucaramanga, Colombia.
Veterinary world
|September 28, 2023
概括
这项研究从肠中分离出乳酸细菌 (LAB),以发现新的益生菌. 几种Enterococcus和Lactococcus菌株对家禽显示出有前途的益生菌潜力.
科学领域:
- 微生物学 微生物学
- 动物科学动物科学
- 益生菌 益生菌 益生菌
背景情况:
- 为家禽开发特定物种的益生菌需要将微生物从它们的自然肠道环境中分离出来.
- 这样可以确保益生菌适应肉和产蛋的实际条件.
研究的目的:
- 为了将乳酸细菌 (LAB) 从肉和产卵的肠道中分离出来.
- 评估这些孤立的LAB菌株的益生菌潜力.
主要方法:
- 乳酸细菌从肉和的小肠粘液中分离出来.
- 分离物被评估为抗低pH值,胆盐和对抗病原细菌的抗药性,如大肠杆菌和沙门氏菌.
- 通过聚合酶链反应和rDNA测序确定了有前途的菌株.
主要成果:
- 获得了150个阳性分离物,其中含有*Enterococcus* spp. 是最普遍的 (76.2%).
- 分子表征确定了*Enterococcus faecium* (56%) 和*Enterococcus faecalis* (44%) 作为关键物种.
- 36个LAB菌株表现出益生菌性质,包括对低pH和胆盐的耐受性,以及对抗活性.
结论:
- 这项研究成功地从肠中分离和表征了36种LAB菌株,这些菌株具有显著的益生菌潜力.
- *Enterococcus faecium*, *Enterococcus avium*, *Enterococcus casseliflavus* 和 *Lactococcus garviae* 被确定为进一步研究的有希望的候选人.
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