在精子生成过程中,SPAG17调解了原蛋白的核转移
Clara Agudo-Rios1,2, Amber Rogers1, Isaiah King1
1Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, United States.
Frontiers in cell and developmental biology
|September 28, 2023
概括
在精子发育过程中,SPAG17蛋白对于从细胞质运输原胺 (PRM1和PRM2) 到细胞核至关重要. 这确保了正确的精子染色质紧缩和男性生育能力.
科学领域:
- 生殖生物学 生殖生物学
- 分子细胞生物学 分子细胞生物学
- 精子发生是精子生成.
背景情况:
- 质氨酸 (PRM1,PRM2) 是精子中关键的核蛋白质,替代了用于染色质凝结的基因组.
- 缺陷的原氨基化会损害男性的生育能力,原因是DNA碎片化增加.
- 有效的原胺核转移对于精子生产至关重要.
研究的目的:
- 为了研究SPAG17在精子生成过程中蛋白胺运输中的作用.
- 为了确定SPAG17是否与蛋白胺核进口相互作用并促进它.
主要方法:
- 近距离结合试验检测SPAG17-原胺相互作用.
- 免疫沉和质谱 (IP/MS) 来验证相互作用.
- 在Spag17淘汰赛小鼠精子中染色和免疫光的染色和染色.
- 在体外核转位检测体细胞中的核转位检测.
主要成果:
- 在细胞质和细胞核中,SPAG17与原胺相互作用.
- 在Spag17淘汰赛中,小鼠显示出异常的原胺和减少的核原胺比率.
- 在淘汰赛丸中,Prm1/Prm2 mRNA或蛋白质水平没有变化.
- 在体内和体外,SPAG17缺乏会影响原胺核转位.
结论:
- SPAG17对于促进原氨酸 (PRM1,PRM2) 运输到精子细胞核至关重要.
- 对于男性生育能力来说,SPAG17在原氨酸贩运中的作用是必不可少的.
- 结果强调SPAG17作为男性不孕症研究的潜在目标.
相关概念视频
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Spermatogenesis
102.6K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.6K
Nuclear Protein Sorting
4.7K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.7K
Nuclear Localization Signals and Import
5.8K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
5.8K
Fertilization
71.1K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
71.1K
Nuclear Export
3.7K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.7K


