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衰老老鼠下尿路功能障碍背后的氧化机制受损

Daisuke Gotoh^1,2, Kazumasa Torimoto², Kenta Onishi²

¹Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States.

American journal of physiology. Renal physiology

|September 28, 2023

概括

老年老鼠由于氧化信号受损而表现出下尿路功能障碍. 这项针对老老鼠模型的研究揭示了与排泄功能障碍相关的分子变化,突出显示了NO.

科学领域:

泌尿器科泌尿器科泌尿器科泌尿器科
老年学是一门学科.
分子生物学分子生物学

背景情况:

衰老与下泌尿道功能障碍 (LUTD) 有关.
氧化 (NO) 在调节膀和尿道功能方面起着至关重要的作用.
了解LUTD与年龄相关的分子变化对于开发向疗法至关重要.

研究的目的:

为了研究老老鼠的膀和尿道活动.
为了检查老老鼠中与氧化 (NO) 相关的分子变化.
建立老年老鼠作为与年龄相关的LUTD的模型.

主要方法:

年轻的 (12 周) 和老化的 (15 个月) 老鼠的比较.
24小时排空测定,清醒囊泡测量 (CMG) 和尿道 perfusion 压力 (UPP) 记录.
评估mRNA表达 (NO,缺血,炎症相关标志物) 和膀和尿道组织中的cGMP水平.

主要成果:

老鼠的非排空收缩增加,排空后的残留体积增加,排空效率降低.
尿道压力降低和高频振荡 (HFO) 幅度在老老鼠中较低.
衰老老鼠表现出膀mRNAHif-1α,Vegf-a,Tgf-β1的水平升高,尿道mRNANo1,Prkg1和cGMP的水平降低.

结论:

老年老鼠表现出与年龄相关的较低尿路功能障碍.
氧化 (NO) 介导的受损发射器功能是与年龄相关的LUTD的一个关键机制.
老鼠作为一个有价值的模型来研究与年龄相关的LUTD的进展.

关键词:

老化的老化衰老的老化破坏者过度活动.氧化氧化的使用方法鼠类鼠类鼠类鼠类鼠类尿动力学尿动力学

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