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转录因子OxyR调节了Corynebacterium glutamicum中硫去除和L-氨酸生物合成的作用
Huanmin Du1,2, Yuting Qi3, Jinfang Qiao4
1Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin, China.
Applied and environmental microbiology
|September 28, 2023
概括
转录因子OxyR通过控制katA和nrdH的表达来调节Corynebacterium glutamicum中的硫硫水平. 这种机制还增强了硫的吸收和L-氨酸的产生.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 硫硫,包括多硫化和有机硫化,在高度下可能对细胞有毒.
- 谷氨酸菌 (Corynebacterium glutamicum) 是一种重要的工业微生物,用于氨基酸的生产,但管理细胞内硫硫的机制尚不清楚.
- OxyR是一种已知的转录因子,对氧化应激和硫硫酸盐有反应.
研究的目的:
- 研究OxyR在调节Corynebacterium glutamicum细胞内硫硫水平中的作用.
- 阐明与硫代谢相关的OxyR影响的特定基因和途径.
- 探索OxyR在优化含硫化合物的生物合成方面的潜力.
主要方法:
- 电泳运动转移试验 (EMSA) 测定OxyR与目标促进体的结合.
- 实时定量PCR (RT-qPCR) 和报告系统用于评估基因表达.
- RNA测序 (RNA-seq) 用于分析全球基因表达变化.
- 代谢分析以量化L-氨酸的积累.
主要成果:
- OxyR直接与katA和nrdH的促进子区域结合,调节它们的表达.
- 过度表达katA和nrdH导致细胞内硫硫水平显著降低.
- OxyR 缺乏导致硫同化途径基因和硫转录因子的下调表达.
- 过度表达OxyR显著增加了C. glutamicum中L-氨酸的积累.
结论:
- OxyR在通过katA和nrdH调节控制C. glutamicum中细胞内硫硫水平方面发挥着至关重要的作用.
- OxyR促进了硫的同化,并增强了L-氨酸生物合成途径.
- OxyR是工程C. glutamicum的一个有前途的目标,作为一种强大的细胞工厂,用于含硫的氨基酸和衍生物.
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