在miR319c和JAW-TCPs之间的双负反循环建立了Arabidopsis thaliana初始叶原始体的生长模式
Naveen Shankar1, Preethi Sunkara1, Utpal Nath1
1Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru, India.
PLoS genetics
|September 28, 2023
概括
微RNA miR319和JAW-TCP转录因子建立了叶子生长模式. 它们相互抑制,将年轻的叶子原始分为不同的繁殖和分化区域.
科学领域:
- 植物发育生物学植物发育生物学
- 分子遗传学 分子遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 微RNA miR319和JAW-TCP转录因子控制了叶子细胞的分化.
- 在Arabidopsis primordia叶子中,MIR319C和JAW-TCPs的互补表达模式尚不清楚.
研究的目的:
- 研究产生MIR319C和JAW-TCP空间表达模式的调节机制.
- 阐明JAW-TCP在调节MIR319C表达和叶子发育中的作用.
主要方法:
- 在Arabidopsis.中分析MIR319C和JAW-TCP表达模式.
- 对JAW-TCPs进行基因操纵 (功能丧失和子宫外表达).
- 染色体免疫沉以检测组织蛋白修饰 (H3K27me3).
- 在MIR319C.中,TCP结合的cis元素的突变发生.
主要成果:
- MIR319C的表达最初是均的,然后在远程下调,与JAW-TCP的外观相吻合.
- 失去JAW-TCPs扩展了MIR319C的远距离表达;宫外TCP活动限制了近距离表达.
- 在MIR319C染色质上,JAW-TCPs存放H3K27me3标记,并且需要完整的cis元素进行抑制.
结论:
- 在MIR319C和JAW-TCPs之间的反循环模式叶原生长.
- 这种调节系统将原始物分为近距离的增殖和远距离的分化区域.
- 通过表观遗传修饰和直接结合,JAW-TCPs通过表观遗传修饰和直接结合来调节MIR319C的抑制.
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