相关实验视频

Updated: Jul 15, 2025

CD Spectroscopy to Study DNA-Protein Interactions

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

与CDCA7-HELLS ICF相关的核细胞重塑复合体和DNA甲基转移酶的共同进化

Hironori Funabiki¹, Isabel E Wassing¹, Qingyuan Jia¹

¹Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, United States.

|September 28, 2023

概括

这项研究揭示了CDCA7,HELLS和DNA甲基转移酶 (DNMTs) 在物种之间共同演变,这表明它们在DNA甲基化维护中发挥着保留作用,这是从早期真核生物中继承的.

科学领域:

遗传学遗传学是一个
进化生物学进化生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

5-甲基细胞氨酸 (5mC) 对于真核生物表观遗传学至关重要,但在进化过程中经常丢失.
SNF2 ATPase HELLS及其激活剂CDCA7对于5mC维持至关重要,它们的突变导致ICF综合征.
DNA甲基转移酶 (DNMTs) 建立和维护DNA甲基化模式.

研究的目的:

为了研究CDCA7,HELLS和DNMT的共同进化模式.
了解DNA甲基化基因丢失和进化史之间的关系.
为了探索CDCA7在DNA甲基化中的保存功能.

主要方法:

对基因存在缺失模式的比较基因组学分析.
在Ecdysozoa中存在-缺席模式 (CoPAP) 的共同进化分析.
保存基因的遗传学分析.

主要成果:

CDCA7,HELLS和DNMT1 (维护甲基转移酶) 在脊椎动物和植物中高度保存,但在其他类中经常被共同丢失.
基因存在缺席模式是非随机的,CDCA7存在与HELLS和DNMT1/DNMT5.5相关.
在CoPAP分析中,发现CDCA7,HELLS,DNMT1和UHRF1.1之间存在共同进化的联系.

结论:

CDCA7可能在依赖HELLS的DNA甲基化维护中发挥着专门的,保存的作用.
CDCA7的丧失可能与DNA甲基化损失有关,或触发替代色素调节机制.
这种复杂的共同进化表明CDCA7在DNA甲基化中的功能具有深厚的进化起源.

关键词:

通过DNA甲基化.这就是ICF综合征.共同进化的共同进化进化生物学是进化的生物学.没有,没有,没有.核酶体重塑器核细胞体重塑器

更多相关视频

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

相关概念视频

Nucleosome Remodeling

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

The Nucleosome Core Particle

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...