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在接受试管婴儿胚胎移植的早期堕胎患者中,H19促进体DNA甲基化水平较低

Lei Han¹, Xianghui Zhang², Xiaohan Wu²

¹Department of Reproductive Medicine, Binzhou Medical University Hospital, Binzhou City, Shandong Province, 256603, China; Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, 401120, China; Department of Reproductive Medicine, Maternal and Child Health Hospital Affiliated to Zunyi Medical University, Zunyi City, Guizhou Province, 563000, China.

Clinical and investigative medicine. Medecine clinique et experimentale

|September 28, 2023

概括

在IVF-ET后的自发流产 (SA) 中,较低的H19促进体DNA甲基化可能与减少的DNA甲基转移酶 (DNMT) 表达有关. 这一发现揭示了早期怀孕丧失机制.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发展生物学发展生物学
生殖医学生殖医学

背景情况:

H19是一种长非编码RNA (lncRNA),对胚胎发育和印记至关重要.
在IVF-ET后自发流产 (SA) 中调查H19促进体DNA甲基化对于了解早期妊娠流产至关重要.

研究的目的:

为了比较SA和正常早期怀孕 (NEP) 群体之间的胆管中的H19促进体DNA甲基化水平.
分析DNA甲基转移酶 (DNMTs) 与SA和NEP中的H19甲基化相关的活动.

主要方法:

双硫酸盐测序用于检测32名SA和32名NEP患者的胆 ?? 囊中的H19促进体DNA甲基化.
实时PCR分析了DNMTT (DNMT1,DNMT3a,DNMT3b) 的mRNA表达.

主要成果:

与NEP组相比,SA组中H19促进体中的7个CpG岛屿中的3个观察到明显较低的甲基化率 (P < 0.01).
两组之间没有发现DNMT1表达的显著差异.
在SA组,DNMT3a和DNMT3bRNA水平明显低于NEP组 (P<0.01).

结论:

在IVF-ET后SA中减少H19促进体DNA甲基化可能与DNMT3a和DNMT3b的表达减少有关.
这些发现表明,潜在的表观遗传机制有助于早孕流产.

关键词:

通过DNA甲基化.这就是H19基因.这是自发流产.印记的基因印记的基因胆 ?? 毛是因为胆 ?? 毛.

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