Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:24

DNA Isolation

39.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Searching for sequence features that control DNA cyclizability.

PNAS nexus·2026
Same author

Intra-operative femoral condyle fractures during primary total knee arthroplasty: Incidence, risk patterns, and early outcomes in an Asian cohort.

Journal of clinical orthopaedics and trauma·2026
Same author

TOP1α-mediated stabilization of CYP82C4 G-quadruplex DNA represses lateral root primordium initiation in Arabidopsis.

Nature plants·2026
Same author

Astrocytes in predictive processing of emotion: Kir4.1 as precision modulator of social interaction.

Acta pharmaceutica Sinica. B·2026
Same author

Probing mechanical selection in diverse eukaryotic genomes through accurate prediction of 3D DNA mechanics.

bioRxiv : the preprint server for biology·2025
Same author

Potential of a newly isolated lytic bacteriophage to control Pseudomonas coronafaciens pv. garcae in coffee plants: Molecular characterization with in vitro and ex vivo experiments.

Enzyme and microbial technology·2024
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
Same journal

An automated workflow for quantifying the formation of synuclein aggregates in human dopaminergic neurons.

Methods (San Diego, Calif.)·2026
查看所有相关文章

相关实验视频

Updated: Jul 15, 2025

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.5K

一种测定DNA灵活性的方法.

Eliz Cirakli1, Aakash Basu2

  • 1Department of Chemistry, Durham University, Durham, UK; Department of Biosciences, Durham University, Durham, UK.

Methods (San Diego, Calif.)
|September 28, 2023
PubMed
概括
此摘要是机器生成的。

科学家们开发了一种更快,低吞吐量的方法来测量DNA机制. 这种技术证实了整合宿主因子 (IHF) 结合部位充当了硬的路障,影响了细菌的基因调节.

关键词:
染色体生物学 染色体生物学循环-seqq 的情况.核子组是核子组中的一个.序列依赖的DNA机制

更多相关视频

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.9K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.1K

相关实验视频

Last Updated: Jul 15, 2025

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.5K
DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.9K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.1K

科学领域:

  • 分子生物学分子生物学
  • 生物物理学的生物物理.
  • 基因组学就是基因组学.

背景情况:

  • 包括可曲性和扭曲刚性在内的DNA机制对于转录和复制等生物过程至关重要.
  • 特定序列的DNA机制可以调节基因表达,形成"机械代码".
  • 在大型基因组区域测量依赖序列的DNA机制以前是具有挑战性的.

研究的目的:

  • 开发一种快速,低吞吐量的方法来测量DNA灵活性,而不需要下一代测序.
  • 验证集成宿主因子 (IHF) 结合位点在细菌促进体中作为机械路障的作用.

主要方法:

  • 开发了一种循环-seq协议的变体,用于低通量DNA灵活性测量.
  • 该方法用于评估大肠杆菌中集成宿主因子 (IHF) 结合位点的DNA机制.

主要成果:

  • 新的循环序列变种可以快速,低吞吐量测量DNA灵活性.
  • 实验验证证证实,IHF结合点充当了刚性路障,阻碍了增强剂-促进剂接触.
  • IHF结合可以缓解这种障碍,调节基因调节.

结论:

  • 开发的方法为研究依赖序列的DNA机制提供了有价值的工具.
  • 这些发现支持IHF结合位点作为大肠杆菌中基因表达的机械调节器的模型.