相关实验视频

Updated: Jul 15, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Rhs NADase效应体及其免疫蛋白是Serratia中的细菌间竞争的可交换媒介

Martin Hagan¹, Genady Pankov¹, Ramses Gallegos-Monterrosa¹

¹School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.

Nature communications

|September 28, 2023

概括

细菌VI型分泌系统 (T6SSs) 使用Rhs效应器与NADase毒素进行竞争. 不相关的免疫蛋白保护这些毒素,显示出灵活的细菌防御策略.

科学领域:

微生物学微生物学
细菌的竞争细菌的竞争
蛋白质分泌系统蛋白质分泌系统

背景情况:

在许多细菌物种中,VI型分泌系统 (T6SS) 对于细菌间的竞争至关重要.
由T6SSs传递的Rhs因子具有有毒的C端域 (CTs) 并与相关的免疫蛋白相关.
孤儿免疫蛋白在与细菌接触时提供对外因子的保护.

研究的目的:

为了研究Rhs1效应因子及其相关免疫蛋白的多样性和功能,来自Serratia marcescens菌株Db10和SJC1036.
阐明NAD(P) +糖酶活性和因无关免疫蛋白抑制效应者的机制.
探索Rhs效应因子和孤儿免疫蛋白在细菌菌株间竞争中的作用.

主要方法:

来自Serratia marcescens菌株的Rhs1效应CT域的比较分析.
结构分析以确定NADase活动的保存域.
功能性测试以评估NAD(P) +糖酶活性和免疫蛋白抑制.
复制自然重组事件以研究效应器域交换.

主要成果:

来自Db10和SJC1036的Rhs1因子具有具有NAD(P) +糖酶活性的独特CT,属于不同的NADase亚组.
不相关的NADase免疫蛋白采用一种共同的机制来抑制效应因子的活动.
实现了CTs的功能交换,并且Db10的孤儿免疫蛋白对SJC1036 NADase.保护.
纳达酶毒素在细菌菌株间竞争中经常被用作武器.

结论:

在细菌菌株间的竞争中,Rhs作用因子和孤儿免疫蛋白被灵活地使用.
该研究揭示了NADase活性和免疫蛋白常见的抑制机制的保存功能要求.
这些发现凸显了T6SS武器的进化适应性,NADase毒素是一个反复出现的主题.

更多相关视频

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

相关概念视频

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

Adherens Junctions

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...