适应商业显微镜的动态全场光学连贯性断层扫描模块允许在体外细胞培养研究的纵向研究
Tual Monfort1,2,3, Salvatore Azzollini1, Jérémy Brogard1
1Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France.
Communications biology
|September 28, 2023
概括
一个新的动态全场光学连贯性断层扫描 (D-FFOCT) 模块可以无标签,长期3D成像活细胞和有机体. 这种先进的成像技术显著提高了生物研究的效率和信号质量.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 显微镜的使用方法
背景情况:
- 无标签成像对于观察活生物样本而不会改变它们的自然状态至关重要.
- 动态全场光学连贯断层扫描 (D-FFOCT) 提供了高分辨率的实时3D成像功能.
- 当前的D-FFOCT系统在长期成像和与标准显微镜设置集成方面可能存在局限性.
研究的目的:
- 介绍一个新的D-FFOCT模块设计,以与商业显微镜无集成.
- 为了实现活生物样本的非侵入性,无标签的纵向3D成像.
- 优化D-FFOCT数据采集和处理,以提高效率和信号质量.
主要方法:
- 开发和整合一个D-FFOCT模块与显微镜和舞台顶部化器.
- 人类诱导的多能干细胞干细胞衍生的视网膜器官和视网膜扩展物的纵向体积成像.
- 为D-FFOCT数据采集,后处理和保存实施一个优化的工作流.
- 开发一种提高D-FFOCT信号与噪声比率的方法.
主要成果:
- 在长时间内证明了视网膜器官和扩展物的非侵入性,无标签的纵向成像.
- 成功地在3D中可视化了细胞过程,包括罗塞特形成,细胞分裂,细胞形状和运动性.
- 与以前的方法相比,实现了数据处理的10倍时间增长.
- 展示了用于快速器官选的增强信号噪声比.
结论:
- 介绍的D-FFOCT模块为研究复杂的生物系统提供了先进的无标签实时成像.
- 优化的工作流程和增强的信号质量显著提高了D-FFOCT的效率和适用性.
- 这项技术具有很大的潜力,可以促进发育生物学,再生医学和疾病建模方面的研究.
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