相关实验视频

Updated: Jul 15, 2025

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

基因组流行病学揭示了在也门的Vibrio cholerae血统之间传播的多药耐药等离子体

Florent Lassalle¹, Salah Al-Shalali², Mukhtar Al-Hakimi²

¹Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK. florent.lassalle@sanger.ac.uk.

Nature microbiology

|September 28, 2023

概括

这就是为什么也门也门也门也门.

科学领域:

微生物学微生物学
基因组学就是基因组学.
流行病学流行病学

背景情况:

自2016年以来,也门面临着全球最大的霍乱疫情.
2018年,Vibrio cholerae中出现了多种药物耐药性 (MDR).
在也门,霍乱的治疗涉及宏胺.

研究的目的:

为了描述在也门循环的Vibrio cholerae基因型.
调查多药耐药性的出现和传播.
了解驱动霍乱菌株抗菌素耐药性的遗传因素.

主要方法:

在也门 (2018-2019) 收集的260个Vibrio cholerae分离物的分析.
霍乱病毒血清组O1 (第七次大流行埃尔托尔血统) 和非-7PET血统的基因组特征.
检测多药耐药性等离子体,包括IncC等离子体和SXT元素.

主要成果:

84%的分离物是V. cholerae血清组O1,7PET系,子系T13.
16%的分离物属于不同的非-7PET系.
T13亚克隆与携带MDR伪化合物转体的Incc等离子体的出现和主导地位与宏化物治疗相吻合.
在非7PET系中检测到MDR等离子体,这表明基因交换.

结论:

在7PET背景中,IncC等离子体和SXT元素同时存在,影响霍乱控制.
基因组流行病学监测对于限制霍乱中MDR传播至关重要.
了解基因交换对于管理V. cholerae的抗菌素耐药性至关重要.

更多相关视频

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Published on: October 8, 2012

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

相关概念视频

Genomic DNA in Prokaryotes

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...

Development of Antibiotic Resistance

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...

Antibiotic Selection

Antibiotic Selection