相关实验视频

Updated: Jul 15, 2025

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

通过下一代测序识别新型HLA-DPA1*01:149等位基因的鉴定

Tiziana Galluccio¹, Raffaella Cerretti^2,3, Annalisa Guagnano¹

¹Department of Oncohematology and Cell and Gene Therapy, Laboratory of Immunogenetics and Transplant, IRCCS Bambin Gesù Pediatric Hospital, Rome, Italy.

|September 29, 2023

概括

一个新的人类白细胞抗原 (HLA) DPA1等位基因,HLA-DPA1*01:149,已被确定. 它与已知等位基因的区别在于,在2号外基因中发生的单个核酸变化.

科学领域:

免疫遗传学免疫遗传学
分子生物学分子生物学
人类白细胞抗原 (HLA) 研究

背景情况:

人类白细胞抗原 (HLA) 系统在免疫反应中起着至关重要的作用.
精确的HLA类型定型对于移植和疾病关联研究至关重要.
新的HLA等位基因发现有助于更全面地了解HLA多态性.

研究的目的:

报告发现和初步表征一种新的HLA-DPA1等位基因.
详细说明区分这种新等位基因与以前识别的特定基因变异.

主要方法:

使用了高分辨率的HLA排版技术.
进行了相关表子的核酸测序.
得到的序列与现有的HLA数据库条目进行比较.

主要成果:

一个新的HLA等位基因被确定,被指定为HLA-DPA1*01:149.
这种新等位基因通过单个核酸替代与HLA-DPA1*01:03:01:05不同.
替代位于HLA-DPA1基因的第2个外显子内.

结论:

鉴定HLA-DPA1*01:149扩大了已知的HLA-DPA1等位基因库.
这一发现强调了持续高分辨率HLA类型识别对于编目遗传多样性的重要性.
进一步的研究可能会探索这种新型基因的潜在功能或临床影响.

关键词:

HLA-DPA1*01:149新增的一种一个新的等位基因.基于序列的打字方式.

更多相关视频

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Published on: August 23, 2024

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....